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Product Info Summary
| SKU: | PA2214 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-HNF6/ONECUT1 Antibody Picoband®
View all HNF-6/ONECUT1 Antibodies
SKU/Catalog Number
PA2214
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HNF6/ONECUT1 Antibody catalog # PA2214. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HNF6/ONECUT1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2214)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg Thimerosal, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human HNF6, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2214 is reactive to ONECUT1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Calculated molecular weight
51023 MW
Background of HNF-6/ONECUT1
One cut homeobox 1 (ONECUT1), also called Hepatocyte nuclear factor 6 (HNF6) is found strong expression in liver and lower expression in testis and skin. ONECUT1 gene is mapped to 15q21.3. This gene encodes a member of the Cut homeobox family of transcription factors. Expression of the encoded protein is enriched in the liver, where it stimulates transcription of liver-expressed genes, and antagonizes glucocorticoid-stimulated gene transcription. This gene may influence a variety of cellular processes including glucose metabolism, cell cycle regulation, and it may also be associated with cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2214 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human HepG2 whole cell, human HUH7 whole cell, rat liver tissue, rat RH35 whole cell, mouse liver tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ONECUT1 using anti-ONECUT1 antibody (PA2214).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human HUH7 whole cell lysates,
Lane 3: rat liver tissue lysates,
Lane 4: rat RH35 whole cell lysates,
Lane 5: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ONECUT1 antigen affinity purified polyclonal antibody (Catalog # PA2214) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ONECUT1 at approximately 55 kDa. The expected band size for ONECUT1 is at 51 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ONECUT1 (Source: Uniprot.org, NCBI)
Gene Name
ONECUT1
Full Name
Hepatocyte nuclear factor 6
Weight
51023 MW
Superfamily
CUT homeobox family
Alternative Names
Hepatocyte nuclear factor 6;HNF-6;One cut domain family member 1;One cut homeobox 1;ONECUT1;HNF6, HNF6A; ONECUT1 HNF-6, HNF6, HNF6A one cut homeobox 1 hepatocyte nuclear factor 6|hepatocyte nuclear factor 6, alpha|one cut domain family member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ONECUT1, check out the ONECUT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ONECUT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HNF6/ONECUT1 Antibody Picoband® (PA2214)
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1 Customer Q&As for Anti-HNF6/ONECUT1 Antibody Picoband®
Question
We are currently using anti-HNF6/ONECUT1 antibody PA2214 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
D. Mitchell
Verified customer
Asked: 2014-10-16
Answer
The anti-HNF6/ONECUT1 antibody (PA2214) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-10-16
