Ptf1a Antibodies

Pancreas transcription factor 1 subunit alpha (Ptf1a)

Transcription factor implicated in the cell fate determination in various organs. Binds to the E-box consensus sequence 5'-CANNTG-3'.

Plays a role in late and early pancreas development and differentiation. Important for determining whether cells allocated to the pancreatic buds continue to pancreatic organogenesis or revert back to duodenal fates.

Gene Information

Mouse
Gene Name:	Ptf1a
Uniprot:	Q9QX98
Entrez:	19213

Family

Belongs to:
No superfamily

Alternative Names

bHLH transcription factor p48; BHLHA29; bHLHa29exocrine pancreas-specific transcription factor p48; Class A basic helix-loop-helix protein 29; p48 DNA-binding subunit of transcription factor PTF1; pancreas specific transcription factor, 1a; pancreas transcription factor 1 subunit alpha; Pancreas-specific transcription factor 1a; PTF1A; PTF1P48; PTF1-p48; PTF1-p48class II bHLH protein PTF1A

Molecular Weight

Mass (kDA):
35.185 kDA

Genomic Context

Mouse
Location:	2|2 A3
Sequence:	2;

Tissue Specificity

Expressed in precursors of pancreatic islets, acini and ducts.

Diseases

• Diabetes Mellitus
• Congenital Absence
• Neoplasms
• Hypoplasia
• Diabetes Mellitus, Insulin-dependent
• Nervousness
• Pancreatic Neoplasm
• Congenital Abnormality
• Pancreatitis
• Pancreatic Diseases
• Malignant Neoplasms
• Congenital Heart Defects

• Malignant Neoplasm Of Pancreas
• Cell Transformation, Neoplastic
• Fetal Growth Retardation
• Immunologic Deficiency Syndromes
• Ectopic Tissue
• Adenocarcinoma
• Atrophy

Pathways

• Pancreas Development
• Cell Differentiation
• Neurogenesis
• Cell Proliferation
• Exocrine Pancreas Development
• Pathogenesis
• Cell Fate Specification
• Cell Cycle
• Cell Development
• Acinar Cell Differentiation
• Transdifferentiation
• Gastrulation
• Pancreas Morphogenesis

• Regeneration
• Cell Fate Determination
• Liver Development
• Cell Cycle Arrest
• Regionalization
• Mitosis
• Neuron Development

PTMs

• Acetylation

• Methylation

Research Areas

• Protein Kinase

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/Ptf1a

Best Uses of the PTF1A Marker

When you start your next research project, it is important to search for a primary anti-Ptf1A antibody. This gene is found in neural pre-cells and has an inverted relationship to pancreatic, ductal, and carcinoma. This protein is an interesting target for therapeutics and is expressed in a variety of tissues, including the pancreas.

Ptf1a is a transcription factor

The PTF1A transcription factor is a cell-type specific transcription factor. It has a limited expression pattern within the developing central nervous system (CNS). It plays an important role by identifying the type of neuron present in the body. It has been shown that it transiently expressed in neural precursors from the spinal cord, retina, and brain. It could be one of the first genes that can be used as a marker to identify cancer-causing tumors.

The PTF1A marker, a multi-modal imaging tool, can be used in a variety experiments. In vitro, Ptf1a-induced miNSCs can display tripotent differentiation properties. These cells can be transplanted into hippocampus-based models of Alzheimer's disease. Ptf1a induced neurons can be used for cognitive function research in mice. In a nest building test, a PTF1a induced neurosphere didn't produce an immunoreactive band. However, mice expressing Rbpj-expression were unable to generate an immunereactive ring in a test of open field ability.

The human nervous sistem is dependent on the PTF1A protein. It is expressed in the nervous system and regulates NSC proliferation and self renewal. Paracrine signaling is activated by the Notch pathway. In vitro, Ptf1areprogrammed hiNSCs became glial cell-like cells and neurons that are immunoreactive to TUJ1.

Recent research has revealed that Ptf1a can stimulate transcription to downstream TFs which regulate NSC specification, homeostasis, and other aspects of NSC regulation. Their experiments show that PTF1a-reprogrammed INSCs are highly effective in transforming fibroblasts to neurospheres. Zfp521, Sox2 & Zfp521 both have tumorigenic capabilities.

It is expressed through neural precursors

Ptf1a is able to reprogramme miNSCs in vivo to differentiate into three cell types. In our study, we transplanted Ptf1a-tagged miNSCs into the hippocampal region of adult mice, and we analyzed them by immunostaining. Most of the miNSCs developed into neurons that expressed Ptf1A markers, while a few became glial cells and GFAP-positive astrocytes.

In a cell cultures model, the reprogramming capability of Ptf1a had been previously demonstrated. MEFs infected to Dox-inducible Ptf1a viruses generated clusters within six days. MEFs infected by wild-type Ptf1a were able to generate a lot of neurospheres, but they failed to express Sox2, Px6, Nestin, or C-Jun-Factin.

The Ptf1A is a crucial helix/loop/helix protein that is abundant in the pancreas. It prefers to form transcription complexes with E proteins. It is vital in determining the fates of acinar or postmitotic neuronal cell fates and is mediated mainly by downstream genes.

It activates Dll1Notch signaling which controls NSC proliferation. DAPT inhibits neurospheres reprogrammed from Ptf1a. It suggests that the Ptf1a pathway promotes iNSC proliferation through a paracrine mechanism. The Ptf1a/Rbpj compound binds the Dll1 enhancer, triggering transcription of Notch effectors.

This TF helps in the specification, homeostasis, and maintenance of NSCs. It is known that it activates multiple genes in six families. These include Sox2, Pou3f2, as well POU domainTFs. In vivo, Sox2 acts as a central regulator in neurogenesis and has been shown directly to reprogram human Fibroblasts into iNSCs.

It is associated in the opposite direction with pancreatic, ductal, adenocarcinoma

The high mortality rates of pancreatic Cancer are largely due to the late stage of diagnosis. Incidence rates and mortality rates closely correlate. Pancreatic cancer is the seventh leading cause for death in both men and women. Over 330,000 people die each year from the disease. High mortality rates are due to the difficulty of diagnosis and the fact only 10% of patients have localized disease.

The progression of pancreatic ductal adenacarcinoma (PDAC) has been found to be inversely associated with genes related to cortisol. The expression of SMAD4 and TP53 was correlated with the incidence of pancreatic adenocarcinoma. PDAC progression can also be attributed to BRCA2 as well as TP53. However, SERPINA6 has inverse relationships with pancreatic adenocarcinoma-related genes.

It is a target in therapeutics

The PTF1A transcription factor is important for early pancreatic development. This gene is directly involved the initiation transcription of the Rbpjl Gene. This gene is frequently underexpressed during pancreatitis. Pancreatic cells develop an acute inflammatory response when PTF1a becomes deficient. The inflammatory response in pancreatic cell may also be caused by the downregulation or inhibition of PTF1a transcriptional activity. Further exploration of this gene's regulatory mechanisms is needed to understand how PTF1a regulates the expression of Rbpjl.