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Product Info Summary
| SKU: | A00006S364 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-PTEN (S364) Antibody
SKU/Catalog Number
A00006S364
Size
100ul
Form
Liquid
Description
Boster Bio Anti-PTEN (S364) Antibody catalog # A00006S364. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PTEN (S364) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00006S364)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide, corresponding to amino acids 350-400 of Human PTEN.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00006S364 is reactive to PTEN in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
47166 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00006S364 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of PTEN using Anti-PTEN (S364) Antibody (A00006S364).
Immunohistochemistry (IHC) analyzes of PTEN (S364) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-PTEN (S364) Antibody A00006S364
Western blot (WB) analysis of PTEN (S364) polyclonal antibody at 1:500 dilutionLane1:HEK293T cell lysate
Lane2:Mouse spleen tissue lysate
Lane3:Rat spleen tissue lysate
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Click image to see more details
Figure 3. Western blotting validation for Anti-PTEN (S364) Antibody A00006S364
Western blot (WB) analysis of PTEN (S364) pAb at 1:500 dilutionLane1:L02 whole cell lysate(40ug)
Lane2:MCF-7 whole cell lysate(40ug)
Lane3:The Brain tissue lysate of Mouse(40ug)
Lane4:The Brain tissue lysate of Rat(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Click image to see more details
Figure 4. Western blotting validation for Anti-PTEN (S364) Antibody A00006S364
Western blot (WB) analysis of PTEN (S364) pAb at 1:500 dilutionLane1:PC12 whole cell lysate(40ug)
Lane2:The kidney tissue lysate of Mouse(40ug)
Lane3:The kidney tissue lysate of Rat(40ug)
Lane4:The lung tissue lysate of Mouse(40ug)
Lane5:The lung tissue lysate of Rat(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For PTEN (Source: Uniprot.org, NCBI)
Gene Name
PTEN
Full Name
Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
Weight
47166 MW
Superfamily
PTEN phosphatase protein family
Alternative Names
Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN;3.1.3.16 PTEN 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC11, PTENbeta, TEP1, PTEN phosphatase and tensin homolog phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN|MMAC1 phosphatase and tensin homolog deleted on chromosome 10|PTENepsilon|mitochondrial PTENalpha|mitochondrial phosphatase and tensin protein alpha|mutated in multiple advanced cancers 1|phosphatase and tensin-like protein|phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN|protein tyrosine phosphatase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTEN, check out the PTEN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTEN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PTEN (S364) Antibody (A00006S364)
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