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Product Info Summary
| SKU: | P00006 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Phospho-PTEN (S380) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00006
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-PTEN (S380) Rabbit Monoclonal Antibody catalog # P00006. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-PTEN (S380) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00006)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HAE-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PTEN
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00006 is reactive to PTEN in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
53 kDa
Calculated molecular weight
47166 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00006 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of Phospho-PTEN (S380) expression in (1) MCF-7 cell treated with alkaline phosphatase lysate; (2) MCF-7 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For PTEN (Source: Uniprot.org, NCBI)
Gene Name
PTEN
Full Name
Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
Weight
47166 MW
Superfamily
PTEN phosphatase protein family
Alternative Names
Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN;3.1.3.16;3.1.3.48;3.1.3.67;Mutated in multiple advanced cancers 1;Phosphatase and tensin homolog;PTEN;MMAC1, TEP1; PTEN 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC11, PTENbeta, TEP1, PTEN phosphatase and tensin homolog phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN|MMAC1 phosphatase and tensin homolog deleted on chromosome 10|PTENepsilon|mitochondrial PTENalpha|mitochondrial phosphatase and tensin protein alpha|mutated in multiple advanced cancers 1|phosphatase and tensin-like protein|phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN|protein tyrosine phosphatase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTEN, check out the PTEN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTEN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-PTEN (S380) Rabbit Monoclonal Antibody (P00006)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Phospho-PTEN (S380) Rabbit Monoclonal Antibody
Question
My colleagues were happy with the WB result of your anti-Phospho-PTEN (S380) Rabbit Monoclonal antibody. However we have seen positive staining in liver cytoplasm using this antibody. Is that expected? Could you tell me where is PTEN supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
From literature, liver does express PTEN. Generally PTEN expresses in cytoplasm. Regarding which tissues have PTEN expression, here are a few articles citing expression in various tissues:
Epithelium, Pubmed ID: 9187108, 9090379
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 15489334
Spleen, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-10-07
Question
We ordered your anti-Phospho-PTEN (S380) Rabbit Monoclonal antibody for WB on lung a few years ago. I am using human, and We want to use the antibody for IF next. We are interested in examining lung as well as spleen in our next experiment. Could give a recommendation on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
I looked at the website and datasheets of our anti-Phospho-PTEN (S380) Rabbit Monoclonal antibody and it seems that P00006 has been tested on human in both WB and IF. Thus P00006 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-05-22
Question
We are currently using anti-Phospho-PTEN (S380) Rabbit Monoclonal antibody P00006 for mouse tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-03-01
Answer
The anti-Phospho-PTEN (S380) Rabbit Monoclonal antibody (P00006) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-01
Question
We have seen staining in mouse liver. What should we do? Is anti-Phospho-PTEN (S380) Rabbit Monoclonal antibody supposed to stain liver positively?
C. Mitchell
Verified customer
Asked: 2013-04-19
Answer
According to literature liver does express PTEN. According to Uniprot.org, PTEN is expressed in intestine, epithelium, spleen, lung, liver, among other tissues. Regarding which tissues have PTEN expression, here are a few articles citing expression in various tissues:
Epithelium, Pubmed ID: 9187108, 9090379
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 15489334
Spleen, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2013-04-19
