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Product Info Summary
| SKU: | M00006-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PTEN Rabbit Monoclonal Antibody
SKU/Catalog Number
M00006-2
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PTEN Rabbit Monoclonal Antibody catalog # M00006-2. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PTEN Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00006-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HBH-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PTEN
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00006-2 is reactive to PTEN in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
54 kDa
Calculated molecular weight
47166 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00006-2 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, rat brain tissue, rat PC-12 whole cell, mouse brain tissue, mouse NIH/3T3 whole cell
IHC: human colon tissue, Rat lung tissue, Human colon cancer tissue, Human tongue cancer tissue, Human liver tissue, Mouse skin tissue
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PTEN using anti-PTEN antibody (M00006-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTEN antigen affinity purified monoclonal antibody (Catalog # M00006-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTEN at approximately 54 kDa. The expected band size for PTEN is at 47 kDa.
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Immunohistochemical analysis of paraffin-embedded human colon, using PTEN Antibody.
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Immunohistochemical analysis of paraffin-embedded Rat lung, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human colon cancer, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human tongue cancer, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human liver, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse skin, using the Antibody at 1:100 dilution.
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Immunofluorescent analysis of Hela cells, using PTEN Antibody.
Protein Target Info & Infographic
Gene/Protein Information For PTEN (Source: Uniprot.org, NCBI)
Gene Name
PTEN
Full Name
Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
Weight
47166 MW
Superfamily
PTEN phosphatase protein family
Alternative Names
Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN;3.1.3.16;3.1.3.48;3.1.3.67;Mutated in multiple advanced cancers 1;Phosphatase and tensin homolog;PTEN;MMAC1, TEP1; PTEN 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC11, PTENbeta, TEP1, PTEN phosphatase and tensin homolog phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN|MMAC1 phosphatase and tensin homolog deleted on chromosome 10|PTENepsilon|mitochondrial PTENalpha|mitochondrial phosphatase and tensin protein alpha|mutated in multiple advanced cancers 1|phosphatase and tensin-like protein|phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN|protein tyrosine phosphatase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTEN, check out the PTEN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTEN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PTEN Rabbit Monoclonal Antibody (M00006-2)
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Customer Q&As
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16 Customer Q&As for Anti-PTEN Rabbit Monoclonal Antibody
Question
Would anti-PTEN Rabbit Monoclonal antibody M00006-2 work for IF with spleen?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
According to the expression profile of spleen, PTEN is highly expressed in spleen. So, it is likely that anti-PTEN Rabbit Monoclonal antibody M00006-2 will work for IF with spleen.
Boster Scientific Support
Answered: 2020-04-14
Question
Can you help my question with product M00006-2, anti-PTEN Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00006-2 anti-PTEN Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-24
Question
We have seen staining in mouse lung. Are there any suggestions? Is anti-PTEN Rabbit Monoclonal antibody supposed to stain lung positively?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
From what I have seen in literature lung does express PTEN. From what I have seen in Uniprot.org, PTEN is expressed in intestine, epithelium, spleen, lung, liver, among other tissues. Regarding which tissues have PTEN expression, here are a few articles citing expression in various tissues:
Epithelium, Pubmed ID: 9187108, 9090379
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 15489334
Spleen, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-02-04
Question
We are currently using anti-PTEN Rabbit Monoclonal antibody M00006-2 for mouse tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
The anti-PTEN Rabbit Monoclonal antibody (M00006-2) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-22
Question
We are interested in using your anti-PTEN Rabbit Monoclonal antibody for male mating behavior studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
I appreciate your inquiry. This M00006-2 anti-PTEN Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-21
Question
See below the WB image, lot number and protocol we used for spleen using anti-PTEN Rabbit Monoclonal antibody M00006-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-01
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for spleen using anti-PTEN Rabbit Monoclonal antibody M00006-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-26
Question
Is a blocking peptide available for product anti-PTEN Rabbit Monoclonal antibody (M00006-2)?
M. Jha
Verified customer
Asked: 2019-09-12
Answer
We do provide the blocking peptide for product anti-PTEN Rabbit Monoclonal antibody (M00006-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-12
Question
I am interested in to test anti-PTEN Rabbit Monoclonal antibody M00006-2 on human spleen for research purposes, then I may be interested in using anti-PTEN Rabbit Monoclonal antibody M00006-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
The products we sell, including anti-PTEN Rabbit Monoclonal antibody M00006-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-06
Question
I see that the anti-PTEN Rabbit Monoclonal antibody M00006-2 works with IF, what is the protocol used to produce the result images on the product page?
K. Parker
Verified customer
Asked: 2018-08-31
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-08-31
Question
My boss were satisfied with the WB result of your anti-PTEN Rabbit Monoclonal antibody. However we have observed positive staining in liver cytoplasm using this antibody. Is that expected? Could you tell me where is PTEN supposed to be expressed?
J. Mangal
Verified customer
Asked: 2017-06-29
Answer
From literature, liver does express PTEN. Generally PTEN expresses in cytoplasm. Regarding which tissues have PTEN expression, here are a few articles citing expression in various tissues:
Epithelium, Pubmed ID: 9187108, 9090379
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 15489334
Spleen, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-06-29
Question
Is there a BSA free version of anti-PTEN Rabbit Monoclonal antibody M00006-2 available?
Verified Customer
Verified customer
Asked: 2017-06-19
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-PTEN Rabbit Monoclonal antibody M00006-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-06-19
Question
I was wanting to use your anti-PTEN Rabbit Monoclonal antibody for IF for human spleen on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human spleen identification?
B. Jones
Verified customer
Asked: 2017-06-16
Answer
As indicated on the product datasheet, M00006-2 anti-PTEN Rabbit Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human spleen in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-16
Question
Is this M00006-2 anti-PTEN Rabbit Monoclonal antibody reactive to the isotypes of PTEN?
G. Li
Verified customer
Asked: 2016-12-22
Answer
The immunogen of M00006-2 anti-PTEN Rabbit Monoclonal antibody is A synthesized peptide derived from human PTEN . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-12-22
Question
We ordered your anti-PTEN Rabbit Monoclonal antibody for IF on epithelium in a previous project. I am using human, and We are going to use the antibody for WB next. My question regards examining epithelium as well as lung in our next experiment. Could give a recommendation on which antibody would work the best for WB?
E. Kulkarni
Verified customer
Asked: 2015-06-05
Answer
I took a look at the website and datasheets of our anti-PTEN Rabbit Monoclonal antibody and it seems that M00006-2 has been tested on human in both IF and WB. Thus M00006-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-06-05
Question
Does M00006-2 anti-PTEN Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Krishna
Verified customer
Asked: 2013-01-09
Answer
It shows on the product datasheet, M00006-2 anti-PTEN Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-01-09
