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Product Info Summary
| SKU: | PB9385 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PTEN Antibody Picoband®
SKU/Catalog Number
PB9385
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PTEN Antibody Picoband® catalog # PB9385. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PTEN Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9385)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PTEN recombinant protein (Position: E91-V403). Human PTEN shares 99.7% amino acid (aa) sequence identity with mouse PTEN.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9385 is reactive to PTEN in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
47 kDa
Calculated molecular weight
47166 MW
Background of PTEN
PTEN is also known as BZS, DEC, CWS1, GLM2, MHAM, TEP1, PTEN1. It is mapped to 10q23.3. This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. The PTEN structure reveals a phosphatase domain that is similar to protein phosphatases but also has an enlarged active site important for the accommodation of the phosphoinositide substrate.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9385 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat, Human
Positive Control
WB: Rat Brain Tissue
Validation Images & Assay Conditions
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Anti-PTEN Picoband antibody, PB9385, Western blotting
All lanes: Anti PTEN (PB9385) at 0.5ug/ml
WB: Rat Brain Tissue Lysate at 50ug
Predicted bind size: 47KD
Observed bind size: 47KD
Protein Target Info & Infographic
Gene/Protein Information For PTEN (Source: Uniprot.org, NCBI)
Gene Name
PTEN
Full Name
Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
Weight
47166 MW
Superfamily
PTEN phosphatase protein family
Alternative Names
Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN;3.1.3.16;3.1.3.48;3.1.3.67;Mutated in multiple advanced cancers 1;Phosphatase and tensin homolog;PTEN;MMAC1, TEP1; PTEN 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC11, PTENbeta, TEP1, PTEN phosphatase and tensin homolog phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN|MMAC1 phosphatase and tensin homolog deleted on chromosome 10|PTENepsilon|mitochondrial PTENalpha|mitochondrial phosphatase and tensin protein alpha|mutated in multiple advanced cancers 1|phosphatase and tensin-like protein|phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN|protein tyrosine phosphatase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTEN, check out the PTEN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTEN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PTEN Antibody Picoband® (PB9385)
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-PTEN Antibody Picoband®
Question
Is a blocking peptide available for product anti-PTEN antibody (PB9385)?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
We do provide the blocking peptide for product anti-PTEN antibody (PB9385). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-28
Question
My team were well pleased with the WB result of your anti-PTEN antibody. However we have observed positive staining in epithelium cytoplasm using this antibody. Is that expected? Could you tell me where is PTEN supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
According to literature, epithelium does express PTEN. Generally PTEN expresses in cytoplasm. Regarding which tissues have PTEN expression, here are a few articles citing expression in various tissues:
Epithelium, Pubmed ID: 9187108, 9090379
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 15489334
Spleen, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-12-25
Question
We are currently using anti-PTEN antibody PB9385 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
The anti-PTEN antibody (PB9385) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-14
Question
I see that the anti-PTEN antibody PB9385 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-01
Question
Here is the WB image, lot number and protocol we used for spleen using anti-PTEN antibody PB9385. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-23
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for spleen using anti-PTEN antibody PB9385. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-08
Question
I have a question about product PB9385, anti-PTEN antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Bhatt
Verified customer
Asked: 2019-09-13
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9385 anti-PTEN antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-13
Question
Does PB9385 anti-PTEN antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
It shows on the product datasheet, PB9385 anti-PTEN antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-13
Question
Will anti-PTEN antibody PB9385 work for WB with spleen?
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
According to the expression profile of spleen, PTEN is highly expressed in spleen. So, it is likely that anti-PTEN antibody PB9385 will work for WB with spleen.
Boster Scientific Support
Answered: 2019-09-05
Question
Is this PB9385 anti-PTEN antibody reactive to the isotypes of PTEN?
C. Evans
Verified customer
Asked: 2019-08-20
Answer
The immunogen of PB9385 anti-PTEN antibody is E.coli-derived human PTEN recombinant protein (Position: E91-V403). Human PTEN shares 99.7% amino acid (aa) sequence identity with mouse PTEN. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-20
Question
Our lab want to know about to test anti-PTEN antibody PB9385 on human spleen for research purposes, then I may be interested in using anti-PTEN antibody PB9385 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Edwards
Verified customer
Asked: 2019-03-11
Answer
The products we sell, including anti-PTEN antibody PB9385, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-03-11
Question
I was wanting to use your anti-PTEN antibody for WB for human spleen on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human spleen identification?
T. Jha
Verified customer
Asked: 2018-08-31
Answer
As indicated on the product datasheet, PB9385 anti-PTEN antibody has been tested for WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human spleen in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-31
Question
We have been able to see staining in human spleen. Are there any suggestions? Is anti-PTEN antibody supposed to stain spleen positively?
Verified Customer
Verified customer
Asked: 2018-06-05
Answer
Based on literature spleen does express PTEN. Based on Uniprot.org, PTEN is expressed in intestine, epithelium, spleen, lung, liver, among other tissues. Regarding which tissues have PTEN expression, here are a few articles citing expression in various tissues:
Epithelium, Pubmed ID: 9187108, 9090379
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 15489334
Spleen, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-06-05
Question
Is there a BSA free version of anti-PTEN antibody PB9385 available?
Verified Customer
Verified customer
Asked: 2017-10-16
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PTEN antibody PB9385 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-10-16
Question
We need using your anti-PTEN antibody for negative regulation of erk1 and erk2 cascade studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-05-30
Answer
I appreciate your inquiry. This PB9385 anti-PTEN antibody is validated on rat brain tissue, tissue lysate. It is guaranteed to work for WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-05-30
