Product Info Summary
SKU: | A08767 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-PSMD3 Antibody
View all Proteasome 19S S3 Antibodies
SKU/Catalog Number
A08767
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-PSMD3 Antibody (Catalog # A08767). Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PSMD3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08767)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human PSMD3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08767 is reactive to PSMD3 in Human
Reconstitution
Calculated molecular weight
60978 MW
Background of Proteasome 19S S3
Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
Farmer A., Submitted (MAY-2003).
Sugano S., Nat. Genet. 36:40-45(2004).
Bennett K.L., Submitted (OCT-2008).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08767 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from K562 cells, using PSMD3 antibody A08767.
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using PSMD3 antibody A08767.
Protein Target Info & Infographic
Gene/Protein Information For PSMD3 (Source: Uniprot.org, NCBI)
Gene Name
PSMD3
Full Name
26S proteasome non-ATPase regulatory subunit 3
Weight
60978 MW
Superfamily
proteasome subunit S3 family
Alternative Names
26S proteasome non-ATPase regulatory subunit 3;26S proteasome regulatory subunit RPN3;26S proteasome regulatory subunit S3;Proteasome subunit p58;PSMD3; PSMD3 P58, RPN3, S3, TSTA2 proteasome 26S subunit, non-ATPase 3 26S proteasome non-ATPase regulatory subunit 3|26S proteasome regulatory subunit RPN3|26S proteasome regulatory subunit S3|proteasome (prosome, macropain) 26S subunit, non-ATPase, 3|proteasome subunit p58|tissue specific transplantation 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PSMD3, check out the PSMD3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PSMD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PSMD3 Antibody (A08767)
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No publications found for A08767
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Customer Q&As
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6 Customer Q&As for Anti-PSMD3 Antibody
Question
Does anti-PSMD3 antibody A08767 work for WB with embryonic kidney?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
According to the expression profile of embryonic kidney, PSMD3 is highly expressed in embryonic kidney. So, it is likely that anti-PSMD3 antibody A08767 will work for WB with embryonic kidney.
Boster Scientific Support
Answered: 2020-03-31
Question
See below the WB image, lot number and protocol we used for embryonic kidney using anti-PSMD3 antibody A08767. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-18
Question
Is there a BSA free version of anti-PSMD3 antibody A08767 available?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-PSMD3 antibody A08767 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-24
Question
We are currently using anti-PSMD3 antibody A08767 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
The anti-PSMD3 antibody (A08767) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-21
Question
I have a question about product A08767, anti-PSMD3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A08767 anti-PSMD3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-24
Question
Is this A08767 anti-PSMD3 antibody reactive to the isotypes of PSMD3?
Verified Customer
Verified customer
Asked: 2018-06-18
Answer
The immunogen of A08767 anti-PSMD3 antibody is Synthesized peptide derived from internal of human PSMD3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-06-18