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Product Info Summary
| SKU: | M02188-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PSMB8 / LMP7 Monoclonal Antibody
View all LMP7/PSMB8 Antibodies
SKU/Catalog Number
M02188-2
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PSMB8 / LMP7 Monoclonal Antibody catalog # M02188-2. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PSMB8 / LMP7 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02188-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AEFI-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Proteasome 20S LMP7 The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02188-2 is reactive to PSMB8 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
27 kDa
Calculated molecular weight
47066 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02188-2 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:100
Validation Images & Assay Conditions
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Western blot analysis of Proteasome 20S LMP7 expression in U937 cell lysate.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For PSMB8 (Source: Uniprot.org, NCBI)
Gene Name
PSMB8
Full Name
Proteasome subunit beta type-8
Weight
47066 MW
Superfamily
peptidase T1B family
Alternative Names
Beta-arrestin-1;Arrestin beta-1;ARRB1;ARR1; PSMB8 ALDD, D6S216, D6S216E, JMP, LMP7, NKJO, PRAAS1, PSMB5i, RING10 proteasome 20S subunit beta 8 proteasome subunit beta type-8|low molecular mass protein 7|low molecular weight protein 7|macropain subunit C13|multicatalytic endopeptidase complex subunit C13|protease component C13|proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7)|proteasome catalytic subunit 3i|proteasome component C13|proteasome subunit Y2|proteasome subunit beta 5i|proteasome subunit beta 8|proteasome-related gene 7|really interesting new gene 10 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PSMB8, check out the PSMB8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PSMB8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PSMB8 / LMP7 Monoclonal Antibody (M02188-2)
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No publications found for M02188-2
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-PSMB8 / LMP7 Monoclonal Antibody
Question
What sample was tested in WB and IHC applications with M02188-2?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of Proteasome 20S LMP7 expression in U937 cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
We are currently using anti-PSMB8 / LMP7 Monoclonal antibody M02188-2 for rat tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
A. Lewis
Verified customer
Asked: 2018-12-04
Answer
The anti-PSMB8 / LMP7 Monoclonal antibody (M02188-2) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-04
Question
My question regarding product M02188-2, anti-PSMB8 / LMP7 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Parker
Verified customer
Asked: 2017-05-31
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02188-2 anti-PSMB8 / LMP7 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-05-31
Question
Please see the WB image, lot number and protocol we used for liver using anti-PSMB8 / LMP7 Monoclonal antibody M02188-2. Please let me know if you require anything else.
R. Collins
Verified customer
Asked: 2014-10-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-10-31
