Anti-PSMB5/MB1 Antibody Picoband®

PSMB5 antibody

Boster Bio Anti-PSMB5/MB1 Antibody Picoband® catalog # A03418-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A03418-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-PSMB5/MB1 Antibody Picoband®

View all PSMB5 Antibodies

SKU/Catalog Number

A03418-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PSMB5/MB1 Antibody Picoband® catalog # A03418-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PSMB5/MB1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03418-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PSMB5/MB1 recombinant protein (Position: M1-P263).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03418-1 is reactive to PSMB5 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

22 kDa

Calculated molecular weight

73109 MW

Background of PSMB5

Proteasome subunit beta type-5 as known as 20S proteasome subunit beta-5 is a protein that in humans is encoded by the PSMB5 gene. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). Multiple transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03418-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -

Positive Control

WB: human Hela whole cell, human HepG2 whole cell, human A549 whole cell, rat liver tissue, rat pancreas tissue, rat testis tissue, rat kidney tissue, mouse liver tissue, mouse testis tissue, mouse kidney tissue
IHC: human breast cancer tissue, human liver cancer tissue, human lymphoma tissue, human ovarian serous adenocarcinoma tissue, human rectal cancer tissue
ICC/IF: MCF-7 cell
FCM: U87 cell

Validation Images & Assay Conditions

Gene/Protein Information For PSMB5 (Source: Uniprot.org, NCBI)

Gene Name

PSMB5

Full Name

Proteasome subunit beta type-5

Weight

73109 MW

Superfamily

peptidase T1B family

Alternative Names

Vacuolar protein sorting-associated protein 4B; Cell migration-inducing gene 1 protein; Suppressor of K (+) transport growth defect 1; Protein SKD1; VPS4B; SKD1; VPS42; MIG1 PSMB5 LMPX, MB1, X proteasome 20S subunit beta 5 proteasome subunit beta type-5|PSX large multifunctional protease X|macropain epsilon chain|multicatalytic endopeptidase complex epsilon chain|proteasome (prosome, macropain) subunit, beta type, 5|proteasome beta 5 subunit|proteasome catalytic subunit 3|proteasome chain 6|proteasome epsilon chain|proteasome subunit MB1|proteasome subunit X|proteasome subunit beta 5|proteasome subunit, beta type, 5|testicular tissue protein Li 153

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PSMB5, check out the PSMB5 Infographic

PSMB5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PSMB5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
A03418-1

100μg

$370
A03418-1-10ug

10μg sample (liquid)

$99
A03418-1-Biotin

100 μg Biotin conjugated

$570
A03418-1-Cy3

100 μg Cy3 conjugated

$570
A03418-1-Dylight488

100 μg Dylight488 conjugated

$570
A03418-1-Dylight550

100 μg Dylight550 conjugated

$570
A03418-1-Dylight594

100 μg Dylight594 conjugated

$570
A03418-1-FITC

100 μg FITC conjugated

$570
A03418-1-HRP

100 μg HRP conjugated

$570
A03418-1-APC

100 μg APC conjugated

$670
A03418-1-PE

100 μg PE conjugated

$670
A03418-1-iFluor647

100 μg iFluor647 conjugated

$670
A03418-1-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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A03418-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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