Anti-PSD3 Antibody Picoband™

Product Name

Anti-PSD3 Antibody Picoband™

View all PSD3 Antibodies

SKU/Catalog Number

A10329-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-PSD3 Antibody Picoband™ catalog # A10329-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PSD3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10329-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PSD3 recombinant protein (Position: N15-E475).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A10329-1 is reactive to PSD3 in Human, Mouse, Rat

Applications

A10329-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

130 kDa

Calculated molecular weight

41409 MW

Background of PSD3

PH and SEC7 domain-containing protein 3 is a protein that in humans is encoded by the PSD3 gene. Predicted to enable guanyl-nucleotide exchange factor activity and phospholipid binding activity. Predicted to be involved in regulation of ARF protein signal transduction and regulation of catalytic activity. Predicted to be located in membrane. Predicted to be active in ruffle membrane.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PSD3 using anti-PSD3 antibody (A10329-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSD3 antigen affinity purified polyclonal antibody (Catalog # A10329-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSD3 at approximately 130 kDa. The expected band size for PSD3 is at 116 kDa.

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Figure 2. Flow Cytometry analysis of RT4 cells using anti-PSD3 antibody (A10329-1).
Overlay histogram showing RT4 cells stained with A10329-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PSD3 Antibody (A10329-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For PSD3 (Source: Uniprot.org, NCBI)

Alternative Names

ADP-ribosylation factor guanine nucleotide factor 6; DKFZp761K1423; EFA6RHepatocellular carcinoma-associated antigen 67; Exchange factor for ADP-ribosylation factor guanine nucleotide factor 6; HCA67Pleckstrin homology and SEC7 domain-containing protein 3; KIAA0942; PH and SEC7 domain-containing protein 3; pleckstrin and Sec7 domain containing 3 PSD3 EFA6D, EFA6R, HCA67 pleckstrin and Sec7 domain containing 3 PH and SEC7 domain-containing protein 3|ADP-ribosylation factor guanine nucleotide factor 6|epididymis tissue protein Li 20mP|exchange factor for ADP-ribosylation factor guanine nucleotide factor 6 D|exchange factor for ARF6 D|hepatocellular carcinoma-associated antigen 67|pleckstrin homology and SEC7 domain-containing protein 3

For more info on PSD3, check out the PSD3 Infographic

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In this infographic, you will see the following information for PSD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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