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Product Info Summary
| SKU: | A01427 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-PSD Antibody Picoband™
SKU/Catalog Number
A01427
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PSD Antibody Picoband™ catalog # A01427. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PSD Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01427)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human PSD recombinant protein (Position: T536-K803).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01427 is reactive to PSD in Human, Mouse, Rat
Applications
A01427 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
130 kDa
Calculated molecular weight
51548 MW
Background of PSD
PSD, which called EFA6A, is 63% identical to EFA6B (PSD4) and contains a Sec7 domain adjacent to a PH domain, followed by a C-terminal coiled-coil motif. This gene is mapped to chromosome 10q24. And it encodes a Plekstrin homology and SEC7 domains-containing protein that functions as a guanine nucleotide exchange factor. The encoded protein regulates signal transduction by activating ADP-ribosylation factor 6. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot,0.1-0.5μg/ml
Direct ELISA,0.1-0.5μg/ml
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PSD using anti-PSD antibody (A01427).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSD antigen affinity purified polyclonal antibody (Catalog # A01427) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSD at approximately 130KD. The expected band size for PSD is at 109KD.
Protein Target Info & Infographic
Gene/Protein Information For PSD (Source: Uniprot.org, NCBI)
Gene Name
PSD
Full Name
PH and SEC7 domain-containing protein 1
Weight
51548 MW
Superfamily
PSD family
Alternative Names
Exchange factor for ARF6; KIAA2011Pleckstrin homology and SEC7 domain-containing protein 1; pleckstrin and Sec7 domain containing; pleckstrin and Sec7 domain protein; PSD1PH and SEC7 domain-containing protein 1; TYLEFA6 PSD EFA6, EFA6A1, TYL, PSD pleckstrin and Sec7 domain containing PH and SEC7 domain-containing protein 1|exchange factor for ADP-ribosylation factor guanine nucleotide factor 6|exchange factor for ARF6 A|pleckstrin homology and SEC7 domain-containing protein 1|pleckstrin-Sec7 domains-containing
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PSD, check out the PSD Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PSD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PSD Antibody Picoband™ (A01427)
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No publications found for A01427
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-PSD Antibody Picoband™
Question
Our lab were content with the WB result of your anti-PSD antibody. However we have been able to see positive staining in right frontal lobe cell membrane. using this antibody. Is that expected? Could you tell me where is PSD supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
According to literature, right frontal lobe does express PSD. Generally PSD expresses in cell membrane. Regarding which tissues have PSD expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9417912, 15164054
Boster Scientific Support
Answered: 2020-04-28
Question
We have observed staining in mouse right frontal lobe. What should we do? Is anti-PSD antibody supposed to stain right frontal lobe positively?
Verified Customer
Verified customer
Asked: 2020-04-10
Answer
According to literature right frontal lobe does express PSD. According to Uniprot.org, PSD is expressed in right frontal lobe, brain, among other tissues. Regarding which tissues have PSD expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9417912, 15164054
Boster Scientific Support
Answered: 2020-04-10
Question
Here is the WB image, lot number and protocol we used for right frontal lobe using anti-PSD antibody A01427. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-27
Question
I was wanting to use your anti-PSD antibody for WB for rat right frontal lobe on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat right frontal lobe identification?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
It shows on the product datasheet, A01427 anti-PSD antibody has been tested for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat right frontal lobe in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-13
Question
My lab would like to test anti-PSD antibody A01427 on rat right frontal lobe for research purposes, then I may be interested in using anti-PSD antibody A01427 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
The products we sell, including anti-PSD antibody A01427, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-05
Question
Would A01427 anti-PSD antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
You can see on the product datasheet, A01427 anti-PSD antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-25
Question
We are currently using anti-PSD antibody A01427 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
The anti-PSD antibody (A01427) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-31
Question
I see that the anti-PSD antibody A01427 works with WB, what is the protocol used to produce the result images on the product page?
A. Miller
Verified customer
Asked: 2019-12-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-24
Question
Can you help my question with product A01427, anti-PSD antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01427 anti-PSD antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-08
Question
Is this A01427 anti-PSD antibody reactive to the isotypes of PSD?
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
The immunogen of A01427 anti-PSD antibody is E. coli-derived human PSD recombinant protein (Position: T536-K803). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-05
Question
Does anti-PSD antibody A01427 work for WB with right frontal lobe?
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
According to the expression profile of right frontal lobe, PSD is highly expressed in right frontal lobe. So, it is likely that anti-PSD antibody A01427 will work for WB with right frontal lobe.
Boster Scientific Support
Answered: 2019-05-13
Question
We purchased anti-PSD antibody for ELISA on brain a few months ago. I am using mouse, and I plan to use the antibody for WB next. Our lab want to know about examining brain as well as right frontal lobe in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-04-17
Answer
I viewed the website and datasheets of our anti-PSD antibody and I see that A01427 has been validated on mouse in both ELISA and WB. Thus A01427 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-04-17
Question
We want using your anti-PSD antibody for regulation of arf protein signal transduction studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-11-15
Answer
I appreciate your inquiry. This A01427 anti-PSD antibody is tested on rat brain tissue, mouse brain. It is guaranteed to work for ELISA, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-11-15
Question
Is a blocking peptide available for product anti-PSD antibody (A01427)?
C. Zhao
Verified customer
Asked: 2015-02-16
Answer
We do provide the blocking peptide for product anti-PSD antibody (A01427). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-02-16
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right frontal lobe using anti-PSD antibody A01427. Let me know if you need anything else.
E. Rodriguez
Verified customer
Asked: 2013-12-11
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-12-11
Question
Do you have a BSA free version of anti-PSD antibody A01427 available?
B. Li
Verified customer
Asked: 2013-02-19
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PSD antibody A01427 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-02-19
