PSD Antibodies

PH and SEC7 domain-containing protein 1 (PSD)

Guanine nucleotide exchange factor for ARF6 (PubMed:23603394). Induces cytoskeletal remodeling (By similarity).

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Gene Information

Human
Gene Name:	PSD
Uniprot:	A5PKW4
Entrez:	5662

Family

Belongs to:
PSD family

Alternative Names

Exchange factor for ARF6; KIAA2011Pleckstrin homology and SEC7 domain-containing protein 1; pleckstrin and Sec7 domain containing; pleckstrin and Sec7 domain protein; PSD1PH and SEC7 domain-containing protein 1; TYLEFA6

Molecular Weight

Mass (kDA):
109.543 kDA

Genomic Context

Human
Location:	10q24.32
Sequence:	10; NC_000010.11 (102402617..102420164, complement)

Tissue Specificity

Isoform 2 is expressed in the brain.

Subcellular Localizations

Cell membrane. Cell projection, ruffle membrane. Cleavage furrow. Distributed uniformly on the plasma membrane, as well as throughout the cytoplasm during metaphase. Subsequently concentrated at patches in the equatorial region at the onset of cytokinesis, and becomes distributed in the equatorial region concurrent with cleavage furrow ingression. In later cytokinesis phases, fades away from the cleavage furrow and becomes uniformly distributed throughout the plasma membrane.

Diseases

• Glaucoma
• Depressive Disorder
• Intraocular Pressure Disorder
• Cerebrovascular Accident
• Glaucoma, Open-angle
• Nervousness
• Hypertensive Disease
• Disorder Of The Optic Nerve
• Neoplasms
• Ocular Hypertension
• Malignant Neoplasms
• Primary Open Angle Glaucoma
• Major Depressive Disorder

• Tissue Adhesions
• Creutzfeldt-jakob Disease
• Ischemia
• Dementia
• Pain
• Alzheimer's Disease
• Schizophrenia

Interacting Proteins

• Usp9x

Pathways

• Localization
• Synaptic Transmission
• Transport
• Pathogenesis
• Flight
• Cell Adhesion
• Translation
• Endocytosis
• Penile Erection
• Secretion
• Brain Development
• Aging
• Coagulation

• Protein Phosphorylation
• Sensitization
• Wound Healing
• Cell Death
• Reflex
• Rna Interference
• Hypersensitivity

PTMs

• Phosphorylation
• Cleavage
• Ribosylation
• Dephosphorylation
• Oxidation
• Methylation
• Myristoylation
• Reduction
• Palmitoylation
• Decarboxylation

• Ubiquitination
• Nitration
• Biotinylation
• Glycosylation
• Acetylation
• Carboxylation
• Iodination
• Amination
• Formylation
• Deglycosylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/PSD

Best Uses Of The PSD Marker

The PSD Marker protein is a highly useful and unique protein that can be used for many biological research applications. It is positioned optimally next to glutamate receptor signalling complexes. It can be used in cell culture as well as ELISA. The MPP2 is located next to these complexes making it an excellent PSD indicator. But how do we use it?

MPP2 is ideally located next to glutamate-receptor signalling complexes

MPP2 is located optimally next to glutamate-receptor signalling complexes. This complex is involved in synaptogenesis as well as synaptic plasticity. This protein interacts cytoplasmically with the NMDA receptor subunits' cytoplasmic tails and shaker-type sodium channels. It affects the ratio of inhibitory to excitatory synapses in hippocampal neurons and may regulate the intracellular trafficking of ADR1B. It is also implicated in the regulation of AMPA-type glutamate receptor immobilization at postsynaptic density.

It is a PSD indicator

PSD-95 and tSynGAP are a pair of protein markers demonstrating a developmentally regulated subcellular expression pattern. Both proteins markers are expressed at PSD both in the late and early stages of development. However they are absent from the cytosolic fraction. These results suggest PSD 95 could be an important marker for the initial stages of PSD maturation. Boster Bio provides both markers in their product line. Additionally, a selection antibodies are also available.

The immunoblotting of PSD-95 with the tSynGAP protein allowed for expression. This protein was found in two subcellular fractions: the cytosol, and the PSD. The PSD-95 ratio was then calculated. In the first two weeks following birth, b and a-isoforms were significantly increased in the cells. A2 isoforms were also found to be expressed in the cytosol and the PSD at similar levels. However, tSynGAP could not be detected in either the NSM or SNP fractions.

It can be used for ELISA

Boster Bio's PSD marker is an RNA Polymerase II (rpoD), primer that detects a specific mRNA. The marker was used to identify a protein of interest. It is highly specific for rpoD, and can be used in ELISA. The Boster Bio PSD marker can be purchased in single-use or multiple-use formats.

It can be used for cell culture

Boster Bio's PSD marker is an antibody that targets neuron-specific Enolase. We compared mRNA expression in cortical cells to the PSD protein. We also determined median neurite lengths by immunostaining cells for antibodies against neuronal-specific Enolase (NPD).

It can be used as a cell culture marker

The PSD gene can be found in mesenchymal and non-stem stem cells. We first cultivated SSC-like cells in 5% CO2 at 37°C to determine if Boster Bio's PSD marker could be used as a cell line marker. These cells were then identified through the expression levels of CD24 and DDX4, UCHL1, ZBTB16. The cells were then incubated in this medium for 4 days to analyze their proliferation and viability.