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Product Info Summary
| SKU: | A11762 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-PRRC2C Antibody Picoband®
SKU/Catalog Number
A11762
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PRRC2C Antibody Picoband® catalog # A11762. Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PRRC2C Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11762)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PRRC2C recombinant protein (Position: H2357-D2808).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A11762 is reactive to PRRC2C in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
290 kDa, 94 kDa
Calculated molecular weight
316.911kDa
Background of PRRC2C
Proline-rich coiled-coil 2C (PRRC2C) is proposed to be involved in protein C-terminus binding and contains a conserved BAT2 N-terminus domain.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11762 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human PC-3 whole cell, human K562 whole cell, human HEL whole cell
ICC/IF: HELA cell
FCM: U251 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PRRC2C using anti-PRRC2C antibody (A11762).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human HEL whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRRC2C antigen affinity purified polyclonal antibody (Catalog # A11762) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRRC2C at approximately 290,94 kDa. The expected band size for PRRC2C is at 317 kDa.
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Figure 2. IF analysis of PRRC2C using anti-PRRC2C antibody (A11762).
PRRC2C was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PRRC2C Antibody (A11762) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of U251 cells using anti-PRRC2C antibody (A11762).
Overlay histogram showing U251 cells stained with A11762 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRRC2C Antibody (A11762, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For PRRC2C (Source: Uniprot.org, NCBI)
Gene Name
PRRC2C
Full Name
Protein PRRC2C
Weight
316.911kDa
Alternative Names
Protein eva-1 homolog A; Protein FAM176A; Transmembrane protein 166; EVA1A; FAM176A; TMEM166; SP24 PRRC2C BAT2-iso, BAT2D1, BAT2L2, XTP2 proline rich coiled-coil 2C protein PRRC2C|BAT2 domain containing 1|BAT2 domain-containing protein 1|HBV X-transactivated gene 2 protein|HBV XAg-transactivated protein 2|HBxAg transactivated protein 2|HLA-B-associated transcript 2-like 2|proline-rich and coiled-coil-containing protein 2C|protein BAT2-like 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRRC2C, check out the PRRC2C Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRRC2C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PRRC2C Antibody Picoband® (A11762)
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