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Product Info Summary
| SKU: | M13414 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | ICC, WB |
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Product info
Product Name
Anti-PRR7 Purified Monoclonal Antibody
SKU/Catalog Number
M13414
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PRR7 Purified Monoclonal Antibody (Catalog# M13414). Tested in WB, ICC application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-PRR7 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M13414)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
TRAP3/10
Isotype
Mouse IgG2a
Immunogen
Recombinant C-terminal half of the intracellular domain of human PRR7/TRAP3 (amino acids 126-253). The mouse monoclonal antibody TRAP3/10 recognizes an epitope located in the C-terminal part of the intracellular domain of PRR7/TRAP3 (amino acids 126-253 of human PRR7 / TRAP3), a 28 kDa proline-rich membrane protein presumably associated with NMDA receptor complex.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
As in case with other anti-CD170 antibodies, this antibody cross-reacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.
Reactive Species
M13414 is reactive to PRR7 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
30930 MW
Background of PRR7
PRR7/TRAP3 (proline-rich 7, transmembrane adaptor protein 3) is a 28 kDa transmembrane adaptor protein ubiquitously expressed at low level (most in brain). Its amino acid sequence is extremely conserved among mammalian and other species. PRR7/TRAP3 contains potential palmitoylation motif and is found in lipid rafts. It is a part of the complex postsynaptic density fraction in neurons and associates with PSD-95, NMDA receptor and probably other proteins. The intracellular domain of PRR7/TRAP3 contains several tyrosines, a proline-rich sequence, and a C-terminal PDZ-binding motif. So far nothing is known about function of this protein. It may be involved in regulation of some receptor signaling and in formation of neurologic and immunologic synapse.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M13414 is guaranteed for ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry: 10 μg/ml; cell culture fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton-X100.
Western blotting: 1 μg/ml; positive control: murine brain lysate (red. Laemmli buffer).
Validation Images & Assay Conditions
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Figure 1. Immunocytochemistry staining of PRR7 using Anti-PRR7 Purified Monoclonal Antibody (M13414).
Immunocytochemistry staining of HEK-293 cells cotransfected with PRR7 / TRAP3 (red) and GFP-PSD-95 (green). PRR7 / TRAP3 detected by monoclonal antibody TRAP3/10. DNA visualized by DAPI (blue).
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Figure 2. Western blotting validation for Anti-PRR7 Purified Monoclonal Antibody M13414
Western blotting detection of PRR7 / TRAP3 in murine brain lysate using the monoclonal antibody TRAP3/10.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For PRR7 (Source: Uniprot.org, NCBI)
Gene Name
PRR7
Full Name
Proline-rich protein 7
Weight
30930 MW
Alternative Names
TRAP3 PRR7 proline rich 7, synaptic proline-rich protein 7|synaptic proline-rich membrane protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRR7, check out the PRR7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRR7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PRR7 Purified Monoclonal Antibody (M13414)
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