Product Info Summary
SKU: | M02434 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PRP19 PRPF19 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02434
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PRP19 PRPF19 Rabbit Monoclonal Antibody catalog # M02434. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PRP19 PRPF19 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02434)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FEC-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PRP19
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02434 is reactive to PRPF19 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
24 kDa
Calculated molecular weight
55181 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02434 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
IHC: human cervix cancer tissue
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of PRP19 expression in (1) HepG2 cell lysate; (2) NIH/3T3 cell lysate.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human cervix cancer, using PRP19 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For PRPF19 (Source: Uniprot.org, NCBI)
Gene Name
PRPF19
Full Name
Pre-mRNA-processing factor 19
Weight
55181 MW
Superfamily
WD repeat PRP19 family
Alternative Names
Pre-mRNA-processing factor 19 ;6.3.2.- ;Nuclear matrix protein 200 ;PRP19/PSO4 homolog ;hPso4 ;Senescence evasion factor ;PRPF19 ;NMP200 , PRP19 , SNEV ; PRPF19 NMP200, PRP19, PSO4, SNEV, UBOX4, hPSO4 pre-mRNA processing factor 19 pre-mRNA-processing factor 19|PRP19/PSO4 homolog|PRP19/PSO4 pre-mRNA processing factor 19 homolog|RING-type E3 ubiquitin transferase PRP19|nuclear matrix protein 200|nuclear matrix protein NMP200 related to splicing factor PRP19|psoralen 4|senescence evasion factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRPF19, check out the PRPF19 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRPF19: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PRP19 PRPF19 Rabbit Monoclonal Antibody (M02434)
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Customer Q&As
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3 Customer Q&As for Anti-PRP19 PRPF19 Rabbit Monoclonal Antibody
Question
Will anti-PRP19 Rabbit Monoclonal antibody M02434 work on bovine IF with frontal cortex?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
Our lab technicians have not tested anti-PRP19 Rabbit Monoclonal antibody M02434 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-PRP19 Rabbit Monoclonal antibody M02434 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine frontal cortex in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-06
Question
I see that the anti-PRP19 Rabbit Monoclonal antibody M02434 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-07-27
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-07-27
Question
We are currently using anti-PRP19 Rabbit Monoclonal antibody M02434 for mouse tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2017-12-04
Answer
The anti-PRP19 Rabbit Monoclonal antibody (M02434) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-12-04