Anti-Protocadherin FAT2 (FAT Atypical Cadherin 2) Monoclonal Antibody

Product Name

Anti-Protocadherin FAT2 (FAT Atypical Cadherin 2) Monoclonal Antibody

View all FAT2 Antibodies

SKU/Catalog Number

M04835

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Protocadherin FAT2 (FAT Atypical Cadherin 2) Monoclonal Antibody (Catalog # M04835). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Protocadherin FAT2 (FAT Atypical Cadherin 2) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04835)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: 8C5

Isotype

IgG1, kappa

Immunogen

Purified recombinant human FAT2 fusion protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M04835 is reactive to FAT2 in Human

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

479317 MW

Background of FAT2

Recognizes a protein of 480kDa, which is identified as FAT2. The cadherins represent a family of Ca2+-dependent adhesion molecules that function to mediate cell-to-cell binding that is critical for the maintenance of structure and morphogenesis. Cadherins each contain a large extracellular domain at the N-terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity. The relatively short C-terminal intracellular domain interacts with a variety of cytoplasmic proteins, including -catenin, to regulate cadherin function. The cadherin superfamily includes cadherins, protocadherins, desmogleins and desmocollins. FAT2 (FAT tumor suppressor homolog 2) is a single-pass type I membrane protein that belongs to the protocadherin subfamily of cadherins. FAT2 contains one Laminin G-like domain, two EGF-like domains and 32 cadherin domains and is believed to function as a cell adhesion molecule, controlling cell proliferation and playing an important role in cerebellum development.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M04835 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For FAT2 (Source: Uniprot.org, NCBI)

Alternative Names

Protocadherin Fat 2;hFat2;Cadherin family member 8;Multiple epidermal growth factor-like domains protein 1;Multiple EGF-like domains protein 1;FAT2;CDHF8, KIAA0811, MEGF1; FAT2 CDHF8, CDHR9, HFAT2, MEGF1, SCA45 FAT atypical cadherin 2 protocadherin Fat 2|FAT tumor suppressor homolog 2|cadherin family member 8|cadherin-related family member 9|multiple EGF-like domains protein 1|multiple epidermal growth factor-like domains 1|multiple epidermal growth factor-like domains protein 1|protocadherin FAT2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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