Click image to see more details
Product Info Summary
| SKU: | M00044-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Prothrombin Antibody Picoband® (monoclonal, 4G13G9)
View all Coagulation Factor II/Thrombin Antibodies
SKU/Catalog Number
M00044-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Prothrombin Antibody Picoband® (monoclonal, 4G13G9) catalog # M00044-2. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Prothrombin Antibody Picoband® (monoclonal, 4G13G9) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00044-2)
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
4G13G9
Isotype
Mouse IgG2b
Immunogen
E. coli-derived human Prothrombin recombinant protein (Position: Y97-R124).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M00044-2 is reactive to F2 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
70 kDa
Calculated molecular weight
14377 MW
Background of Coagulation Factor II/Thrombin
F2 (Coagulation Factor II), also known as thrombin, is a serine protease that in humans is encoded by the F2 gene. This gene for human prothrombin (F2) was assigned to chromosome 11p11-q12 by analysis of a panel of somatic cell hybrid DNAs and by in situ hybridization, using both cDNA and genomic probes. The activated thrombin enzyme plays an important role in hemostasis and thrombosis: it converts fibrinogen to fibrin for blood clot formation, stimulates platelet aggregation, and activates coagulation factors V, VIII (F8), and XIII (F13A1). Thrombin also inhibits coagulation by activating protein C.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00044-2 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Positive Control
WB: human HepG2 whole cell
IHC: human liver cancer tissue, human colorectal cancer tissue, human ovarian cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Prothrombin using anti-Prothrombin antibody (M00044-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Prothrombin antigen affinity purified monoclonal antibody (Catalog # M00044-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Prothrombin at approximately 70 kDa. The expected band size for Prothrombin is at 70 kDa.
Click image to see more details
Figure 2. IHC analysis of Prothrombin using anti-Prothrombin antibody (M00044-2).
Prothrombin was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Prothrombin Antibody (M00044-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Prothrombin using anti-Prothrombin antibody (M00044-2).
Prothrombin was detected in a paraffin-embedded section of human colorectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Prothrombin Antibody (M00044-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Prothrombin using anti-Prothrombin antibody (M00044-2).
Prothrombin was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Prothrombin Antibody (M00044-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For F2 (Source: Uniprot.org, NCBI)
Gene Name
F2
Full Name
Prothrombin
Weight
14377 MW
Superfamily
peptidase S1 family
Alternative Names
Signal transducer and activator of transcription 1-alpha/beta; Transcription factor ISGF-3 components p91/p84; STAT1 F2 PT, RPRGL2, THPH1 coagulation factor II, thrombin prothrombin|prepro-coagulation factor II|prothrombin B-chain|thrombin factor II
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on F2, check out the F2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for F2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Prothrombin Antibody Picoband® (monoclonal, 4G13G9) (M00044-2)
Hello CJ!
No publications found for M00044-2
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Prothrombin Antibody Picoband® (monoclonal, 4G13G9)?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Prothrombin Antibody Picoband® (monoclonal, 4G13G9)
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
