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Product Info Summary
| SKU: | M00044 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-Prothrombin F2 Monoclonal Antibody
View all Coagulation Factor II/Thrombin Antibodies
SKU/Catalog Number
M00044
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Prothrombin F2 Monoclonal Antibody catalog # M00044. Tested in WB, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Prothrombin F2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00044)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACBD-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Prothrombin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00044 is reactive to F2 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
83, 88 kDa
Calculated molecular weight
55391 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00044 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IP 1:50
Positive Control
WB: human serum
Validation Images & Assay Conditions
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Western blot analysis of Prothrombin expression in human serum lysate.
Protein Target Info & Infographic
Gene/Protein Information For F2 (Source: Uniprot.org, NCBI)
Gene Name
F2
Full Name
Prothrombin
Weight
55391 MW
Superfamily
peptidase S1 family
Alternative Names
Caspase-8;CASP-8;3.4.22.61;Apoptotic cysteine protease;Apoptotic protease Mch-5;CAP4;FADD-homologous ICE/ced-3-like protease;FADD-like ICE;FLICE;ICE-like apoptotic protease 5;MORT1-associated ced-3 homolog;MACH;Caspase-8 subunit p18;Caspase-8 subunit p10;CASP8;MCH5; F2 PT, RPRGL2, THPH1 coagulation factor II, thrombin prothrombin|prepro-coagulation factor II|prothrombin B-chain|thrombin factor II
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on F2, check out the F2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for F2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Prothrombin F2 Monoclonal Antibody (M00044)
Hello CJ!
M00044 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Heparin Impairs Angiogenesis through Inhibition of MicroRNA-10b
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Prothrombin F2 Monoclonal Antibody
Question
I see that the anti-Prothrombin Monoclonal antibody M00044 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-24
Question
We have been able to see staining in human right lobe of liver. Are there any suggestions? Is anti-Prothrombin Monoclonal antibody supposed to stain right lobe of liver positively?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
According to literature right lobe of liver does express F2. According to Uniprot.org, F2 is expressed in right lobe of liver, blood, liver mammary gland, liver, urine, plasma, cerebrospinal fluid, among other tissues. Regarding which tissues have F2 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 14962227
Cerebrospinal fluid, Pubmed ID: 19838169
Liver, Pubmed ID: 15489334, 19159218, 24275569
Liver, and Mammary gland, Pubmed ID: 14702039
Plasma, Pubmed ID: 14760718, 16335952
Urine, Pubmed ID: 8073540
Boster Scientific Support
Answered: 2020-04-06
Question
I was wanting to use your anti-Prothrombin Monoclonal antibody for WB for human urine on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human urine identification?
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
As indicated on the product datasheet, M00044 anti-Prothrombin Monoclonal antibody has been tested for IP, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human urine in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-18
Question
Our lab were satisfied with the WB result of your anti-Prothrombin Monoclonal antibody. However we have been able to see positive staining in blood secreted using this antibody. Is that expected? Could you tell me where is F2 supposed to be expressed?
K. Jackson
Verified customer
Asked: 2020-03-16
Answer
According to literature, blood does express F2. Generally F2 expresses in secreted, extracellular space. Regarding which tissues have F2 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 14962227
Cerebrospinal fluid, Pubmed ID: 19838169
Liver, Pubmed ID: 15489334, 19159218, 24275569
Liver, and Mammary gland, Pubmed ID: 14702039
Plasma, Pubmed ID: 14760718, 16335952
Urine, Pubmed ID: 8073540
Boster Scientific Support
Answered: 2020-03-16
Question
My question regarding product M00044, anti-Prothrombin Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00044 anti-Prothrombin Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-24
Question
Would anti-Prothrombin Monoclonal antibody M00044 work for WB with urine?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
According to the expression profile of urine, F2 is highly expressed in urine. So, it is likely that anti-Prothrombin Monoclonal antibody M00044 will work for WB with urine.
Boster Scientific Support
Answered: 2019-08-12
Question
Do you have a BSA free version of anti-Prothrombin Monoclonal antibody M00044 available?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Prothrombin Monoclonal antibody M00044 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-28
Question
Would M00044 anti-Prothrombin Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-04-03
Answer
It shows on the product datasheet, M00044 anti-Prothrombin Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-04-03
Question
I am looking for to test anti-Prothrombin Monoclonal antibody M00044 on human urine for research purposes, then I may be interested in using anti-Prothrombin Monoclonal antibody M00044 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Yang
Verified customer
Asked: 2019-01-11
Answer
The products we sell, including anti-Prothrombin Monoclonal antibody M00044, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-01-11
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for urine using anti-Prothrombin Monoclonal antibody M00044. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-03-08
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-03-08
Question
Is a blocking peptide available for product anti-Prothrombin Monoclonal antibody (M00044)?
M. Dhar
Verified customer
Asked: 2017-11-22
Answer
We do provide the blocking peptide for product anti-Prothrombin Monoclonal antibody (M00044). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-11-22
Question
Our lab used your anti-Prothrombin Monoclonal antibody for WB on plasma in the past. I am using human, and We intend to use the antibody for IP next. I am interested in examining plasma as well as liver in our next experiment. Do you have any suggestion on which antibody would work the best for IP?
K. Yang
Verified customer
Asked: 2016-03-01
Answer
I viewed the website and datasheets of our anti-Prothrombin Monoclonal antibody and I see that M00044 has been tested on human in both WB and IP. Thus M00044 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-03-01
Question
See below the WB image, lot number and protocol we used for urine using anti-Prothrombin Monoclonal antibody M00044. Please let me know if you require anything else.
N. Johnson
Verified customer
Asked: 2015-06-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-06-02
Question
We are currently using anti-Prothrombin Monoclonal antibody M00044 for human tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
E. Bhatt
Verified customer
Asked: 2013-08-21
Answer
The anti-Prothrombin Monoclonal antibody (M00044) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-08-21
Question
Is this M00044 anti-Prothrombin Monoclonal antibody reactive to the isotypes of F2?
Z. Thomas
Verified customer
Asked: 2013-04-29
Answer
The immunogen of M00044 anti-Prothrombin Monoclonal antibody is A synthesized peptide derived from human Prothrombin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-04-29
