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Product Info Summary
| SKU: | A09399 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, ICC, WB |
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Product info
Product Name
Anti-Protein orai-3 ORAI3 Antibody
SKU/Catalog Number
A09399
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Protein orai-3 ORAI3 Antibody (Catalog # A09399). Tested in ELISA, WB, ICC, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ORAI3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Protein orai-3 ORAI3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09399)
Host
Rabbit
Contents
ORAI3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ORAI3 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human ORAI3. The immunogen is located within the first 50 amino acids of ORAI3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI2.
Reactive Species
A09399 is reactive to ORAI3 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
31499 MW
Background of ORAI3
Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09399 is guaranteed for ELISA, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ORAI3 antibody can be used for detection of ORAI3 by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and Immunohistochemistry starting at 2 μg/mL
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ORAI3 in A20 cell lysate with ORAI3 antibody at (A) 1
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Immunocytochemistry of ORAI3 in A20 cells with ORAI3 antibody at 10 μg/mL.
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Immunohistochemistry of ORAI3 in mouse spleen tissue with ORAI3 antibody at 2 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For ORAI3 (Source: Uniprot.org, NCBI)
Gene Name
ORAI3
Full Name
Protein orai-3
Weight
31499 MW
Superfamily
Orai family
Alternative Names
TMEM142C, TMEM142C, Protein orai-3, Transmembrane protein 142C ORAI3 TMEM142C ORAI calcium release-activated calcium modulator 3 protein orai-3|transmembrane protein 142C
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ORAI3, check out the ORAI3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ORAI3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Protein orai-3 ORAI3 Antibody (A09399)
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