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Product Info Summary
| SKU: | A00513 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Protein delta homolog 1 DLK1 Antibody
View all Pref-1/DLK1/FA1 Antibodies
SKU/Catalog Number
A00513
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Protein delta homolog 1 DLK1 Antibody (Catalog # A00513). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
DLK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Protein delta homolog 1 DLK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00513)
Host
Rabbit
Contents
DLK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
DLK1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human DLK1. The immunogen is located within amino acids 260 - 310 of DLK1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least four isoforms of DLK1 are known to exist.
Reactive Species
A00513 is reactive to DLK1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
41300 MW
Background of Pref-1/DLK1/FA1
The Delta-like 1 homolog (DLK1) is a transmembrane protein containing six epidermal growth factor repeats. It is involved in the differentiation of several cell types, including adipocytes and is also thought to be a tumor suppressor. The DLK1 gene is one of several imprinted genes located in a region on chromosome 14q32; certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14). DLK1 is expressed from the paternal allele; a polymorphism within this gene has been associated with child and adolescent obesity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00513 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
DLK1 antibody can be used for detection of DLK1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of HepG2 in 293 cell lysate with DLK1 antibody at (A) 1 and (B) 2 μg/mL.
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Immunocytochemistry of DLK1 in HepG2 cells with DLK1 antibody at 2.5 μg/ml.
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Immunofluorescence of DLK1 in HepG2 cells with DLK1 antibody at 20 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For DLK1 (Source: Uniprot.org, NCBI)
Gene Name
DLK1
Full Name
Protein delta homolog 1
Weight
41300 MW
Alternative Names
DLK, Protein delta homolog 1, pG2, DLK-1 DLK1 DLK, DLK-1, Delta1, FA1, PREF1, Pref-1, ZOG, pG2 delta like non-canonical Notch ligand 1 protein delta homolog 1|delta-like 1 homolog|fetal 1|preadipocyte factor 1|secredeltin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DLK1, check out the DLK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DLK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Protein delta homolog 1 DLK1 Antibody (A00513)
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