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Product Info Summary
| SKU: | A03499-2 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-CEBP Delta Antibody
View all CEBP Delta Antibodies
SKU/Catalog Number
A03499-2
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-CEBP Delta Antibody (Catalog # A03499-2). Tested in ELISA, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-CEBP Delta Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03499-2)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to the amino terminus of mouse C/EBP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03499-2 is reactive to CEBPD in Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Observed Molecular Weight
42 kDa
Calculated molecular weight
93886 MW
Background of CEBP Delta
This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. C/EBP proteins are DNA-binding proteins that are important transcriptional activators in the regulation of genes involved in immune and inflammatory responses and may play an important role in the regulation of the several genes associated with activation and/or differentiation of macrophages. Members of the C/EBP transcription factor family are related by a high degree of amino acid sequence identity to the basic leucine zipper DNA-binding domain and show distinct but overlapping patterns of tissue- and stage-restricted expression. Osada et al. identified the consensus binding site for the family as RTTGCGYAAY (R = A or G, and Y = C or T). Phosphorylation of C/EBPd may increase binding activity, whereas phosphorylation of the a and b family members may decrease binding affinity. C/EBPd is a nuclear protein that binds DNA as a dimer and can form stable heterodimers with all other C/EBP family members.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03499-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:4,000 - 1:9,000
IHC: 1:2000
IP: 0.8 – 1.5 µg/ml
WB: 1:1,000 - 1:15,000
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CEBPD using anti-CEBPD antibody (A03499-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEBPD antigen affinity purified polyclonal antibody (Catalog # A03499-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CEBPD.
Click image to see more details
Figure 2. Western blot analysis of CEBPD using anti-CEBPD antibody (A03499-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEBPD antigen affinity purified polyclonal antibody (Catalog # A03499-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CEBPD.
Protein Target Info & Infographic
Gene/Protein Information For CEBPD (Source: Uniprot.org, NCBI)
Gene Name
CEBPD
Full Name
CCAAT/enhancer-binding protein delta
Weight
93886 MW
Superfamily
bZIP family
Alternative Names
CEBP Delta, C/EBP related protein 3, CCAAT/enhancer binding protein delta, CEBP D, CEBPD, CELF, Crp 3, Crp3, NF IL6 beta, Nuclear factor NF IL6 beta CEBPD C/EBP-delta, CELF, CRP3, NF-IL6-beta CCAAT enhancer binding protein delta CCAAT/enhancer-binding protein delta|CCAAT/enhancer binding protein (C/EBP), delta|c/EBP delta|nuclear factor NF-IL6-beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CEBPD, check out the CEBPD Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CEBPD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CEBP Delta Antibody (A03499-2)
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Customer Q&As
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4 Customer Q&As for Anti-CEBP Delta Antibody
Question
We are currently using anti-CEBP Delta antibody A03499-2 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
The anti-CEBP Delta antibody (A03499-2) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-24
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for female gonad using anti-CEBP Delta antibody A03499-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-08
Question
Would anti-CEBP Delta antibody A03499-2 work for WB with female gonad?
Verified Customer
Verified customer
Asked: 2019-04-08
Answer
According to the expression profile of female gonad, CEBPD is highly expressed in female gonad. So, it is likely that anti-CEBP Delta antibody A03499-2 will work for WB with female gonad.
Boster Scientific Support
Answered: 2019-04-08
Question
Will anti-CEBP Delta antibody A03499-2 work on pig IP with female gonad?
Verified Customer
Verified customer
Asked: 2017-12-14
Answer
Our lab technicians have not validated anti-CEBP Delta antibody A03499-2 on pig. You can run a BLAST between pig and the immunogen sequence of anti-CEBP Delta antibody A03499-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig female gonad in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-12-14
