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Product Info Summary
| SKU: | M08095 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Proteasome 20S beta 7 (PSMB7) Mouse Monoclonal Antibody [Clone ID: OTI1E1]
SKU/Catalog Number
M08095
Size
100 µl
Description
Boster Bio PSMB7 (Proteasome 20S beta 7 ) mouse monoclonal antibody, clone OTI1E1 (formerly 1E1). Catalog# M08095. Tested in IHC, WB. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-Proteasome 20S beta 7 (PSMB7) Mouse Monoclonal Antibody [Clone ID: OTI1E1] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08095)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI1E1
Isotype
IgG1
Immunogen
Human recombinant protein fragment corresponding to amino acids 58-277 of human PSMB7 (NP_002790) produced in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M08095 is reactive to PSMB7 in Human, Monkey, Mouse, Rat
Reconstitution
Observed Molecular Weight
29.8 kDa
Calculated molecular weight
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08095 is guaranteed for IHC, WB Boster Guarantee
Positive Control
Validation Images & Assay Conditions
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Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMB7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMB7.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PSMB7 monoclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For PSMB7 (Source: Uniprot.org, NCBI)
Gene Name
PSMB7
Full Name
Proteasome subunit beta type-7
Weight
Superfamily
peptidase T1B family
Alternative Names
Macropain Chain Z; Proteasome Subunit Z; PSMB7 PSMB7 Z proteasome 20S subunit beta 7 proteasome subunit beta type-7|epididymis secretory sperm binding protein|macropain chain Z|multicatalytic endopeptidase complex chain Z|proteasome (prosome, macropain) subunit, beta type, 7|proteasome catalytic subunit 2|proteasome subunit Z|proteasome subunit alpha|proteasome subunit beta 7|proteasome subunit beta2|protein serine kinase c17
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PSMB7, check out the PSMB7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PSMB7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Proteasome 20S beta 7 (PSMB7) Mouse Monoclonal Antibody [Clone ID: OTI1E1] (M08095)
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No publications found for M08095
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Proteasome 20S beta 7 (PSMB7) Mouse Monoclonal Antibody [Clone ID: OTI1E1]
Question
I have a question about product M08095, anti-Proteasome 20S Beta 2 Subunit antibody (Monoclonal, MCP165). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-05-05
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M08095 anti-Proteasome 20S Beta 2 Subunit antibody (Monoclonal, MCP165), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-05-05
Question
I was wanting to use your anti-Proteasome 20S Beta 2 Subunit antibody (Monoclonal, MCP165) for WB for human embryonic kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human embryonic kidney identification?
Verified Customer
Verified customer
Asked: 2019-12-17
Answer
You can see on the product datasheet, M08095 anti-Proteasome 20S Beta 2 Subunit antibody (Monoclonal, MCP165) has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human embryonic kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-17
Question
Would anti-Proteasome 20S Beta 2 Subunit antibody (Monoclonal, MCP165) M08095 work for WB with embryonic kidney?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
According to the expression profile of embryonic kidney, PSMB7 is highly expressed in embryonic kidney. So, it is likely that anti-Proteasome 20S Beta 2 Subunit antibody (Monoclonal, MCP165) M08095 will work for WB with embryonic kidney.
Boster Scientific Support
Answered: 2019-07-31
Question
We are currently using anti-Proteasome 20S Beta 2 Subunit antibody (Monoclonal, MCP165) M08095 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-02-05
Answer
The anti-Proteasome 20S Beta 2 Subunit antibody (Monoclonal, MCP165) (M08095) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-02-05
Question
Is a blocking peptide available for product anti-Proteasome 20S Beta 2 Subunit antibody (Monoclonal, MCP165) (M08095)?
Verified Customer
Verified customer
Asked: 2017-09-01
Answer
We do provide the blocking peptide for product anti-Proteasome 20S Beta 2 Subunit antibody (Monoclonal, MCP165) (M08095). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-09-01
Question
See attached the WB image, lot number and protocol we used for embryonic kidney using anti-Proteasome 20S Beta 2 Subunit antibody (Monoclonal, MCP165) M08095. Please let me know if you require anything else.
R. Li
Verified customer
Asked: 2014-11-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-11-28
Question
Do you have a BSA free version of anti-Proteasome 20S Beta 2 Subunit antibody (Monoclonal, MCP165) M08095 available?
T. Singh
Verified customer
Asked: 2014-10-23
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Proteasome 20S Beta 2 Subunit antibody (Monoclonal, MCP165) M08095 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-10-23
