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Product Info Summary
| SKU: | A07705-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Proteasome 20S beta 6/PSMB6 Antibody Picoband®
View all Proteasome 20S beta 6 Antibodies
SKU/Catalog Number
A07705-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Proteasome 20S beta 6/PSMB6 Antibody Picoband® catalog # A07705-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Proteasome 20S beta 6/PSMB6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07705-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PSMB6 recombinant protein (Position: A50-D201).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07705-1 is reactive to PSMB6 in Human, Monkey, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
22 kDa
Calculated molecular weight
49229 MW
Background of Proteasome 20S beta 6
Proteasome subunit beta type-6 also known as 20S proteasome subunit beta-1 (based on systematic nomenclature) is a protein that in humans is encoded by the PSMB6 gene. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. The encoded protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07705-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat, Monkey
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human Caco-2 whole cell, human Hela whole cell, monkey COS-7 whole cell, human A549 whole cell, human Jurkat whole cell, human MCF-7 whole cell, rat liver tissue, rat brain tissue, rat lung tissue, rat testis tissue, mouse liver tissue, mouse brain tissue, mouse lung tissue, mouse testis tissue
FCM: A431 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Proteasome 20S Beta 6/PSMB6 using anti-Proteasome 20S Beta 6/PSMB6 antibody (A07705-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: monkey COS-7 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: human Jurkat whole cell lysates,
Lane 6: human MCF-7 whole cell lysates,
Lane 7: rat liver tissue lysates,
Lane 8: rat brain tissue lysates,
Lane 9: rat lung tissue lysates,
Lane 10: rat testis tissue lysates,
Lane 11: mouse liver tissue lysates,
Lane 12: mouse brain tissue lysates,
Lane 13: mouse lung tissue lysates,
Lane 14: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Proteasome 20S Beta 6/PSMB6 antigen affinity purified polyclonal antibody (Catalog # A07705-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Proteasome 20S Beta 6/PSMB6 at approximately 22 kDa. The expected band size for Proteasome 20S Beta 6/PSMB6 is at 22 kDa.
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Figure 2. Flow Cytometry analysis of A431 cells using anti-Proteasome 20S Beta 6/PSMB6 antibody (A07705-1).
Overlay histogram showing A431 cells stained with A07705-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Proteasome 20S Beta 6/PSMB6 Antibody (A07705-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For PSMB6 (Source: Uniprot.org, NCBI)
Gene Name
PSMB6
Full Name
Proteasome subunit beta type-6
Weight
49229 MW
Superfamily
peptidase T1B family
Alternative Names
Protein Hook homolog 3; h-hook3; Hhk3; HOOK3 PSMB6 DELTA, LMPY, Y proteasome 20S subunit beta 6 proteasome subunit beta type-6|PSY large multifunctional protease Y|macropain delta chain|multicatalytic endopeptidase complex delta chain|proteasome (prosome, macropain) subunit, beta type, 6|proteasome catalytic subunit 1|proteasome delta chain|proteasome subunit Y|proteasome subunit beta 6|proteasome subunit beta1|proteasome subunit delta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PSMB6, check out the PSMB6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PSMB6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Proteasome 20S beta 6/PSMB6 Antibody Picoband® (A07705-1)
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