Anti-Prostate Specific Antigen (PSA) Monoclonal Antibody

Product Name

Anti-Prostate Specific Antigen (PSA) Monoclonal Antibody

View all Kallikrein 3/PSA Antibodies

SKU/Catalog Number

M01505

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Prostate Specific Antigen (PSA) Monoclonal Antibody (Catalog # M01505). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Cat.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Prostate Specific Antigen (PSA) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01505)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: 1A7

Isotype

IgG1, kappa

Immunogen

PSA (p30) from human sperm plasma

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M01505 is reactive to KLK3 in Human, Cat

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

28741 MW

Background of Kallikrein 3/PSA

Recognizes a single protein of 33-34kDa, identified as the prostate specific antigen (PSA). This monoclonal antibody is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia. This monoclonal antibody makes an excellent pair with MAb A67-B/E3 for PSA tests.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M01505 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For KLK3 (Source: Uniprot.org, NCBI)

Alternative Names

Prostate-specific antigen;PSA;3.4.21.77;Gamma-seminoprotein;Seminin;Kallikrein-3;P-30 antigen;Semenogelase;KLK3;APS; KLK3 APS, KLK2A1, PSA, hK3 kallikrein related peptidase 3 prostate-specific |P-30 |gamma-seminoprotein|kallikrein-3|semenogelase|seminin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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