Human Prostate Specific Antigen (PSA) OneStep ELISA Kit

Product Name

Human Prostate Specific Antigen (PSA) OneStep ELISA Kit

See all Kallikrein 3/PSA products

SKU/Catalog Number

EK7080

Size

96 wells/kit

*Question: How many samples can I assay/run in this kit?

Description

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human PSA. 96 wells/kit.

Storage & Handling

Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Prostate Specific Antigen (PSA) OneStep ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7080)

Clonality of Antibodies

See Datasheet for details

Sensitivity

0.5 ng/ml

Assay Range

1-32 ng/ml

Cross-Reactivity

There is no detectable cross-reactivity.

Reactive Species

EK7080 is reactive to KLK3 in Human samples

Validated Sample Types

Serum

Application Guarantee

EK7080 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Description	Quantity	Volume	Buffers
Anti-Human PSA Pre-coated 96-well strip microplate	1	8 strips of 12 wells	Anti- PSA monoclonal antibody, Polystyrene micro-well plate
Human PSA Standards (S0~S5)	6	1ml	PSA (0, 1, 2, 8, 16, 32) ng/ml, 0.02M PBS, 20% new-born calf serum, from natural protein
HRP Conjugated anti-Human PSA antibody	1	6ml	HRP Conjugated anti-Human PSA antibody, 0.02M PBS, 20% new-born calf serum, 0.01% azophloxine, from mouse monoclonal antibody
Controls	2	1ml	PSA, 0.02M PBS, 20% new-born calf serum
Color Developing Reagent A	1	7ml	Contains 11m mol/L H2O2
Color Developing Reagent B	1	7ml	Contains 2m mol/L TMB
Stop Solution	1	7ml	2mol/L Dilute sulphuric acid
Plate Sealers	2	Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Data Example Images

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