Product Info Summary
SKU: | M01178 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Prohibitin PHB Rabbit Monoclonal Antibody
View all Prohibitin Antibodies
SKU/Catalog Number
M01178
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Prohibitin PHB Rabbit Monoclonal Antibody catalog # M01178. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Prohibitin PHB Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01178)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DIF-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Prohibitin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01178 is reactive to PHB in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
30 kDa
Calculated molecular weight
29804 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01178 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:30
FC 1:30
Positive Control
WB: human Ramos whole cell, human Caco-2 whole cell, human 293T whole cell, human A549 whole cell, rat brain tissue, rat lung tissue, mouse brain tissue, mouse lung tissue
IHC: human bladder cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PHB using anti-PHB antibody (M01178).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Ramos whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat lung tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHB antigen affinity purified monoclonal antibody (Catalog # M01178) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHB at approximately 30 kDa. The expected band size for PHB is at 30 kDa.
Click image to see more details
Figure 2. IHC analysis of PMB using anti-PMB antibody (M01178).
PMB was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PMB Antibody (M01178) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For PHB (Source: Uniprot.org, NCBI)
Gene Name
PHB
Full Name
Prohibitin
Weight
29804 MW
Superfamily
prohibitin family
Alternative Names
Prohibitin;PHB; PHB HEL-215, HEL-S-54e1, PHB prohibitin prohibitin|epididymis luminal protein 215|epididymis secretory sperm binding protein Li 54e
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PHB, check out the PHB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PHB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Prohibitin PHB Rabbit Monoclonal Antibody (M01178)
Hello CJ!
M01178 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Positional and expressive alteration of prohibitin during the induced differentiation of human hepatocarcinoma SMMC-7721 cells
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