Click image to see more details
Product Info Summary
| SKU: | M02352 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Procalcitonin (4C8.H6.D4) Calca Monoclonal Antibody
SKU/Catalog Number
M02352
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Procalcitonin (4C8.H6.D4) Calca Monoclonal Antibody (Catalog # M02352). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Procalcitonin (4C8.H6.D4) Calca Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02352)
Host
Mouse
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Monoclonal
Clone Number
Clone: 4C8.H6.D4 IgG1 lambda
Isotype
IgG1 lambda
Immunogen
Procalcitonin Antibody was produced in mice prepared by repeated immunizations with full-length recombinant human Procalcitonin protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M02352 is reactive to CALCA in Human
Reconstitution
Calculated molecular weight
13948 MW
Background of CALCA
Anti-Procalcitonin antibody detects human Procalcitonin. Procalcitonin is a peptide hormone mainly produced by the C cells of the thyroid and certain endocrine cells of the lung. Under normal expression conditions, procalcitonin is immediately cleaved into three specific fragments, a N terminal residue, calcitonin and katacalcin. Levels of unprocessed procalcitonin rise significantly after bacterial infection, trauma or shock. This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02352 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: User optimized
WB: 1:1,000 - 1:2,000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Procalcitonin expression in Procalcitonin Protein (lane 2), MW (lane 1). Procalcitonin at 13.9KD was detected using mouse anti-Procalcitonin monoclonal antibody (Catalog # M02352) at NEAT overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
Click image to see more details
Western blot analysis of Procalcitonin expression in rProcalcitonin (lane 1). Procalcitonin at 13.9KD was detected using mouse anti-Procalcitonin monoclonal antibody (Catalog # M02352) at 1:1000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1001).
Protein Target Info & Infographic
Gene/Protein Information For CALCA (Source: Uniprot.org, NCBI)
Gene Name
CALCA
Full Name
Calcitonin
Weight
13948 MW
Superfamily
calcitonin family
Alternative Names
procalcitonin, calcitonin isoform CT preproprotein, calcitonin-related polypeptide alpha, CT, KC, CGRP, CALC1, CGRP1, CGRP-I CALCA CALC1, CGRP, CGRP-I, CGRP-alpha, CGRP1, CT, KC, PCT calcitonin related polypeptide alpha calcitonin|calcitonin gene-related peptide 1|alpha-type CGRP|calcitonin 1|calcitonin gene-related peptide I|calcitonin/calcitonin-related polypeptide, alpha|katacalcin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CALCA, check out the CALCA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CALCA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Procalcitonin (4C8.H6.D4) Calca Monoclonal Antibody (M02352)
Hello CJ!
No publications found for M02352
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Procalcitonin (4C8.H6.D4) Calca Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Procalcitonin (4C8.H6.D4) Calca Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
13 Customer Q&As for Anti-Procalcitonin (4C8.H6.D4) Calca Monoclonal Antibody
Question
I was wanting to use your anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody for WB for human pheochromocytoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human pheochromocytoma identification?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
As indicated on the product datasheet, M02352 anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human pheochromocytoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-19
Question
Is a blocking peptide available for product anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody (M02352)?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
We do provide the blocking peptide for product anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody (M02352). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-31
Question
Is there a BSA free version of anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody M02352 available?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody M02352 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-24
Question
My colleagues were satisfied with the WB result of your anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody. However we have been able to see positive staining in dorsal root ganglion secreted. using this antibody. Is that expected? Could you tell me where is CALCA supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
According to literature, dorsal root ganglion does express CALCA. Generally CALCA expresses in secreted. Regarding which tissues have CALCA expression, here are a few articles citing expression in various tissues:
Pheochromocytoma, Pubmed ID: 1318039
Thyroid carcinoma, Pubmed ID: 6610687
Boster Scientific Support
Answered: 2019-11-08
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pheochromocytoma using anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody M02352. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-17
Question
We are currently using anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody M02352 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
The anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody (M02352) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-24
Question
Does M02352 anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-04-22
Answer
You can see on the product datasheet, M02352 anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-04-22
Question
I see that the anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody M02352 works with WB, what is the protocol used to produce the result images on the product page?
A. Mitchell
Verified customer
Asked: 2018-07-20
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-07-20
Question
I have a question about product M02352, anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Taylor
Verified customer
Asked: 2015-04-23
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02352 anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-04-23
Question
Will anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody M02352 work for WB with pheochromocytoma?
J. Jones
Verified customer
Asked: 2014-02-11
Answer
According to the expression profile of pheochromocytoma, CALCA is highly expressed in pheochromocytoma. So, it is likely that anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody M02352 will work for WB with pheochromocytoma.
Boster Scientific Support
Answered: 2014-02-11
Question
Our lab want to know about to test anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody M02352 on human pheochromocytoma for research purposes, then I may be interested in using anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody M02352 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Roberts
Verified customer
Asked: 2014-02-04
Answer
The products we sell, including anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody M02352, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-02-04
Question
See attached the WB image, lot number and protocol we used for pheochromocytoma using anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody M02352. Please let me know if you require anything else.
S. Jones
Verified customer
Asked: 2013-06-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-06-18
Question
We have observed staining in human pheochromocytoma. Do you have any suggestions? Is anti-Procalcitonin (4C8.H6.D4) Monoclonal antibody supposed to stain pheochromocytoma positively?
D. Williams
Verified customer
Asked: 2013-03-12
Answer
From what I have seen in literature pheochromocytoma does express CALCA. From what I have seen in Uniprot.org, CALCA is expressed in dorsal root ganglion, thyroid carcinoma, pheochromocytoma, among other tissues. Regarding which tissues have CALCA expression, here are a few articles citing expression in various tissues:
Pheochromocytoma, Pubmed ID: 1318039
Thyroid carcinoma, Pubmed ID: 6610687
Boster Scientific Support
Answered: 2013-03-12
