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Product Info Summary
| SKU: | PA2182 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-Mannose 6 Phosphate Receptor (Cation independent)/IGF2R Antibody Picoband®
View all IGF-II R/IGF2R Antibodies
SKU/Catalog Number
PA2182
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Mannose 6 Phosphate Receptor (Cation independent)/IGF2R Antibody catalog # PA2182. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Mannose 6 Phosphate Receptor (Cation independent)/IGF2R Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2182)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Mannose 6 Phosphate Receptor(Cation independent), different from the related rat and mouse sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2182 is reactive to IGF2R in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
274 kDa
Calculated molecular weight
274375 MW
Background of IGF-II R/IGF2R
Insulin-like growth factor 2 receptor, also called IGF2R or I-MPR is a protein that in humans is encoded by the IGF2R gene. This gene is mapped to 6q25.3. This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate, although the binding sites for either are located on different segments of the receptor. This receptor functions in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. While the related mouse gene shows exclusive expression from the maternal allele, imprinting of the human gene appears to be polymorphic, with only a minority of individuals showing expression from the maternal allele.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2182 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human Hela whole cell, human U87 whole cell, human HepG2 whole cell, rat PC-12 whole cell, mouse NIH/3T3 whole cell
FCM: HepG2 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IGF2R using anti-IGF2R antibody (PA2182).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U87 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGF2R antigen affinity purified polyclonal antibody (Catalog # PA2182) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGF2R at approximately 274 kDa. The expected band size for IGF2R is at 274 kDa.
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Figure 2. Flow Cytometry analysis of HepG2 cells using anti-IGF2R antibody (PA2182).
Overlay histogram showing HepG2 cells stained with PA2182 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IGF2R Antibody (PA2182, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For IGF2R (Source: Uniprot.org, NCBI)
Gene Name
IGF2R
Full Name
Cation-independent mannose-6-phosphate receptor
Weight
274375 MW
Superfamily
MRL1/IGF2R family
Alternative Names
Cation-independent mannose-6-phosphate receptor;CI Man-6-P receptor;CI-MPR;M6PR;300 kDa mannose 6-phosphate receptor;MPR 300;Insulin-like growth factor 2 receptor;Insulin-like growth factor II receptor;IGF-II receptor;M6P/IGF2 receptor;M6P/IGF2R;CD222;IGF2R;MPRI; IGF2R CD222, CI-M6PR, CIMPR, M6P-R, M6P/IGF2R, MPR 300, MPR1, MPR300, MPRI insulin like growth factor 2 receptor cation-independent mannose-6-phosphate receptor|300 kDa mannose 6-phosphate receptor|CI Man-6-P receptor|IGF-II receptor|M6P/IGF2 receptor|insulin-like growth factor II receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IGF2R, check out the IGF2R Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IGF2R: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Mannose 6 Phosphate Receptor (Cation independent)/IGF2R Antibody Picoband® (PA2182)
Hello CJ!
PA2182 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression
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Customer Q&As
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7 Customer Q&As for Anti-Mannose 6 Phosphate Receptor (Cation independent)/IGF2R Antibody Picoband®
Question
We are currently using anti-Mannose 6 Phosphate Receptor (Cation independent)/IGF2R antibody PA2182 for mouse tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
The anti-Mannose 6 Phosphate Receptor (Cation independent)/IGF2R antibody (PA2182) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-27
Question
I see that the anti-Mannose 6 Phosphate Receptor (Cation independent)/IGF2R antibody PA2182 works with ICC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-24
Question
you antibody to test anti-Mannose 6 Phosphate Receptor (Cation independent)/IGF2R antibody PA2182 on human leukemic t-cell for research purposes, then I may be interested in using anti-Mannose 6 Phosphate Receptor (Cation independent)/IGF2R antibody PA2182 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
The products we sell, including anti-Mannose 6 Phosphate Receptor (Cation independent)/IGF2R antibody PA2182, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-25
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-Mannose 6 Phosphate Receptor (Cation independent)/IGF2R antibody PA2182. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-04
Question
Is this PA2182 anti-Mannose 6 Phosphate Receptor (Cation independent)/IGF2R antibody reactive to the isotypes of IGF2R?
Verified Customer
Verified customer
Asked: 2017-11-09
Answer
The immunogen of PA2182 anti-Mannose 6 Phosphate Receptor (Cation independent)/IGF2R antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Mannose 6 Phosphate Receptor(Cation independent)(2475-2491aa TKLVSFHDDSDEDLLHI), different from the related rat and mouse sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-11-09
Question
Is a blocking peptide available for product anti-Mannose 6 Phosphate Receptor (Cation independent)/IGF2R antibody (PA2182)?
T. Zhang
Verified customer
Asked: 2016-11-28
Answer
We do provide the blocking peptide for product anti-Mannose 6 Phosphate Receptor (Cation independent)/IGF2R antibody (PA2182). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-11-28
Question
Would PA2182 anti-Mannose 6 Phosphate Receptor (Cation independent)/IGF2R antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Miller
Verified customer
Asked: 2016-07-11
Answer
As indicated on the product datasheet, PA2182 anti-Mannose 6 Phosphate Receptor (Cation independent)/IGF2R antibody as been tested on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-07-11
