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Product Info Summary
| SKU: | M00080-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-pro Caspase 9 CASP9 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00080-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-pro Caspase 9 CASP9 Rabbit Monoclonal Antibody catalog # M00080-1. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-pro Caspase 9 CASP9 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00080-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GAH-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human pro Caspase 9
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00080-1 is reactive to CASP9 in Human
Applications
M00080-1 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
46 kDa
Calculated molecular weight
46281 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human colon, using pro Caspase 9 Antibody.
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All lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.
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Western blot analysis of pro Caspase 9 expression in Jurkat cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CASP9 (Source: Uniprot.org, NCBI)
Gene Name
CASP9
Full Name
Caspase-9
Weight
46281 MW
Superfamily
peptidase C14A family
Alternative Names
APAF-3APAF3; apoptotic protease activating factor 3; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; CASP9; CASP-9; caspase 9, apoptosis-related cysteine peptidase; Caspase9; Caspase-9; EC 3.4.22.62; ICE-LAP6CASPASE-9c; ICE-like apoptotic protease 6; MCH6apoptosis-related cysteine protease CASP9 APAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56 caspase 9 caspase-9|ICE-like apoptotic protease 6|apoptotic protease MCH-6|apoptotic protease activating factor 3|caspase 9, apoptosis-related cysteine peptidase|protein phosphatase 1, regulatory subunit 56
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CASP9, check out the CASP9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CASP9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-pro Caspase 9 CASP9 Rabbit Monoclonal Antibody (M00080-1)
Hello CJ!
M00080-1 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
In Vitro Effects of HAS-2 Gene Silencing on the Proliferation and Apoptosis of the MCF-7 Human Breast Cancer Cell Line
In vivo?and?in vitro?evaluation of the cytotoxic effects of Photosan-loaded hollow silica nanoparticles on liver cancer
Chang C, Liu Sp, Fang Ch, He Rs, Wang Z, Zhu Yq, Jiang Sw. Oncol Lett. 2013 Sep;6(3):699-704. Epub 2013 Jul 8. Effects Of Matrine On The Proliferation Of Ht29 Human Colon Cancer Cells And Its Antitumor Mechanism.
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-pro Caspase 9 CASP9 Rabbit Monoclonal Antibody
Question
Is this M00080-1 anti-pro Caspase 9 Rabbit Monoclonal antibody reactive to the isotypes of CASP9?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
The immunogen of M00080-1 anti-pro Caspase 9 Rabbit Monoclonal antibody is A synthesized peptide derived from human pro Caspase 9 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-24
Question
I have attached the WB image, lot number and protocol we used for t-cell using anti-pro Caspase 9 Rabbit Monoclonal antibody M00080-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-24
Question
Is there a BSA free version of anti-pro Caspase 9 Rabbit Monoclonal antibody M00080-1 available?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-pro Caspase 9 Rabbit Monoclonal antibody M00080-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-04
Question
Is a blocking peptide available for product anti-pro Caspase 9 Rabbit Monoclonal antibody (M00080-1)?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
We do provide the blocking peptide for product anti-pro Caspase 9 Rabbit Monoclonal antibody (M00080-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-26
Question
We are currently using anti-pro Caspase 9 Rabbit Monoclonal antibody M00080-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
The anti-pro Caspase 9 Rabbit Monoclonal antibody (M00080-1) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-16
Question
Would M00080-1 anti-pro Caspase 9 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Kulkarni
Verified customer
Asked: 2019-01-10
Answer
You can see on the product datasheet, M00080-1 anti-pro Caspase 9 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-01-10
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for t-cell using anti-pro Caspase 9 Rabbit Monoclonal antibody M00080-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-12-20
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-20
Question
I am interested in to test anti-pro Caspase 9 Rabbit Monoclonal antibody M00080-1 on human t-cell for research purposes, then I may be interested in using anti-pro Caspase 9 Rabbit Monoclonal antibody M00080-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-12-10
Answer
The products we sell, including anti-pro Caspase 9 Rabbit Monoclonal antibody M00080-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-12-10
Question
We have observed staining in human adrenal tissue. Are there any suggestions? Is anti-pro Caspase 9 Rabbit Monoclonal antibody supposed to stain adrenal tissue positively?
Verified Customer
Verified customer
Asked: 2018-06-19
Answer
From what I have seen in literature adrenal tissue does express CASP9. From what I have seen in Uniprot.org, CASP9 is expressed in adrenal tissue, t-cell, stomach cancer, kidney, eye lymph, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CASP9 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Eye, and Lymph, Pubmed ID: 15489334
Kidney, Pubmed ID: 14702039
Stomach cancer, Pubmed ID: 9890966
T-cell, Pubmed ID: 8900201
Boster Scientific Support
Answered: 2018-06-19
Question
Will anti-pro Caspase 9 Rabbit Monoclonal antibody M00080-1 work for WB with t-cell?
Verified Customer
Verified customer
Asked: 2018-01-23
Answer
According to the expression profile of t-cell, CASP9 is highly expressed in t-cell. So, it is likely that anti-pro Caspase 9 Rabbit Monoclonal antibody M00080-1 will work for WB with t-cell.
Boster Scientific Support
Answered: 2018-01-23
Question
I was wanting to use your anti-pro Caspase 9 Rabbit Monoclonal antibody for WB for human t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human t-cell identification?
Verified Customer
Verified customer
Asked: 2017-11-14
Answer
As indicated on the product datasheet, M00080-1 anti-pro Caspase 9 Rabbit Monoclonal antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-11-14
Question
I see that the anti-pro Caspase 9 Rabbit Monoclonal antibody M00080-1 works with WB, what is the protocol used to produce the result images on the product page?
C. Collins
Verified customer
Asked: 2017-07-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-07-06
Question
My question regarding product M00080-1, anti-pro Caspase 9 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Taylor
Verified customer
Asked: 2016-04-01
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00080-1 anti-pro Caspase 9 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-04-01
Question
you antibody using your anti-pro Caspase 9 Rabbit Monoclonal antibody for response to cobalt ion studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
N. Thomas
Verified customer
Asked: 2016-01-25
Answer
We appreciate your inquiry. This M00080-1 anti-pro Caspase 9 Rabbit Monoclonal antibody is validated on jurkat cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-01-25
