Anti-PRMT5 Rabbit Monoclonal Antibody

Product Name

Anti-PRMT5 Rabbit Monoclonal Antibody

View all PRMT5 Antibodies

SKU/Catalog Number

M00635

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-PRMT5 Rabbit Monoclonal Antibody catalog # M00635. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PRMT5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00635)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

DCE-16

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human PRMT5

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00635 is reactive to PRMT5 in Human, Mouse, Rat

Applications

M00635 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

85 kDa

Calculated molecular weight

72684 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:5000-1:10000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50

Validation Images & Assay Conditions

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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.

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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.

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Western blot analysis of PRMT5 expression in HeLa cell lysate.

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Immunohistochemical analysis of paraffin-embedded human testis, using PRMT5 Antibody .

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Immunofluorescent analysis using the Antibody at 1:150 dilution.

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Immunofluorescent analysis using the Antibody at 1:150 dilution.

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Immunofluorescent analysis using the Antibody at 1:50 dilution.

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Immunofluorescent analysis using the Antibody at 1:50 dilution.

Gene/Protein Information For PRMT5 (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.1.1.-; EC 2.1.1.125; Histone-arginine N-methyltransferase PRMT5; HMT1 hnRNP methyltransferase-like 5,72 kDa ICln-binding protein; HRMT1L5; IBP72; Jak-binding protein 1; JBP1; protein arginine methyltransferase 5; protein arginine N-methyltransferase 5; Shk1 kinase-binding protein 1 homolog; skb1 (S. pombe) homolog; SKB1 homolog (S. pombe); SKB1; SKB1HsSKB1 homolog PRMT5 HRMT1L5, HSL7, IBP72, JBP1, SKB1, SKB1Hs protein arginine methyltransferase 5 protein arginine N-methyltransferase 5|72 kDa ICln-binding protein|HMT1 hnRNP methyltransferase-like 5|SKB1 homolog|histone-arginine N-methyltransferase PRMT5|jak-binding protein 1|shk1 kinase-binding protein 1 homolog

For more info on PRMT5, check out the PRMT5 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PRMT5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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