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Product Info Summary
| SKU: | M00635 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PRMT5 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00635
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PRMT5 Rabbit Monoclonal Antibody catalog # M00635. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PRMT5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00635)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DCE-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PRMT5
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00635 is reactive to PRMT5 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
71 kDa
Calculated molecular weight
71,73 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00635 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:10000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: human Hela whole cell, human 293T whole cell, human MCF-7 whole cell, human K562 whole cell, rat spleen tissue, rat brain tissue, mouse spleen tissue, mouse brain tissue
IHC: human testis tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PRMT5 using anti-PRMT5 antibody (M00635).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat spleen tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse spleen tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRMT5 antigen affinity purified monoclonal antibody (Catalog # M00635) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRMT5 at approximately 71 kDa. The expected band size for PRMT5 is at 71,73 kDa.
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Immunohistochemical analysis of paraffin-embedded human testis, using PRMT5 Antibody .
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For PRMT5 (Source: Uniprot.org, NCBI)
Gene Name
PRMT5
Full Name
Protein arginine N-methyltransferase 5
Weight
71,73 kDa
Superfamily
class I-like SAM-binding methyltransferase superfamily
Alternative Names
Protein arginine N-methyltransferase 5;2.1.1.320 ;72 kDa ICln-binding protein;Histone-arginine N-methyltransferase PRMT5;Jak-binding protein 1;Shk1 kinase-binding protein 1 homolog;SKB1 homolog;SKB1Hs;Protein arginine N-methyltransferase 5, N-terminally processed;PRMT5;HRMT1L5, IBP72, JBP1, SKB1; PRMT5 HRMT1L5, HSL7, IBP72, JBP1, SKB1, SKB1Hs protein arginine methyltransferase 5 protein arginine N-methyltransferase 5|72 kDa ICln-binding protein|HMT1 hnRNP methyltransferase-like 5|SKB1 homolog|histone-arginine N-methyltransferase PRMT5|jak-binding protein 1|shk1 kinase-binding protein 1 homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRMT5, check out the PRMT5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRMT5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PRMT5 Rabbit Monoclonal Antibody (M00635)
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No publications found for M00635
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Customer Q&As
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6 Customer Q&As for Anti-PRMT5 Rabbit Monoclonal Antibody
Question
I see that the anti-PRMT5 Rabbit Monoclonal antibody M00635 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-07
Question
Will anti-PRMT5 Rabbit Monoclonal antibody M00635 work for WB with testis?
Verified Customer
Verified customer
Asked: 2019-07-17
Answer
According to the expression profile of testis, PRMT5 is highly expressed in testis. So, it is likely that anti-PRMT5 Rabbit Monoclonal antibody M00635 will work for WB with testis.
Boster Scientific Support
Answered: 2019-07-17
Question
Is a blocking peptide available for product anti-PRMT5 Rabbit Monoclonal antibody (M00635)?
Verified Customer
Verified customer
Asked: 2019-01-18
Answer
We do provide the blocking peptide for product anti-PRMT5 Rabbit Monoclonal antibody (M00635). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-01-18
Question
Is this M00635 anti-PRMT5 Rabbit Monoclonal antibody reactive to the isotypes of PRMT5?
J. Li
Verified customer
Asked: 2019-01-18
Answer
The immunogen of M00635 anti-PRMT5 Rabbit Monoclonal antibody is A synthesized peptide derived from human PRMT5. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-01-18
Question
I was wanting to use your anti-PRMT5 Rabbit Monoclonal antibody for WB for mouse testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse testis identification?
Verified Customer
Verified customer
Asked: 2018-08-06
Answer
You can see on the product datasheet, M00635 anti-PRMT5 Rabbit Monoclonal antibody has been tested for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-06
Question
Would M00635 anti-PRMT5 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Edwards
Verified customer
Asked: 2013-08-01
Answer
You can see on the product datasheet, M00635 anti-PRMT5 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-08-01
