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Product Info Summary
| SKU: | A01386-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-NAT1 Antibody Picoband®
SKU/Catalog Number
A01386-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NAT1 Antibody Picoband® catalog # A01386-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NAT1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01386-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human NAT1, different from the related mouse sequence by six amino acids, and from the related rat sequence by five amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01386-1 is reactive to NAT1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
34 kDa
Calculated molecular weight
33899 MW
Background of NAT1
N-acetyltransferase 1 (arylamine N-acetyltransferase) is a protein that in humans is encoded by the NAT1 gene. This gene is one of two arylamine N-acetyltransferase (NAT) genes in the human genome, and is orthologous to the mouse and rat Nat2 genes. The enzyme encoded by this gene catalyzes the transfer of an acetyl group from acetyl-CoA to various arylamine and hydrazine substrates. This enzyme helps metabolize drugs and other xenobiotics, and functions in folate catabolism. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01386-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: A431 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NAT1 using anti-NAT1 antibody (A01386-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
lane 1: A431 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAT1 antigen affinity purified polyclonal antibody (Catalog # A01386-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NAT1 at approximately 34KD. The expected band size for NAT1 is at 34KD.
Protein Target Info & Infographic
Gene/Protein Information For NAT1 (Source: Uniprot.org, NCBI)
Gene Name
NAT1
Full Name
Arylamine N-acetyltransferase 1
Weight
33899 MW
Superfamily
arylamine N-acetyltransferase family
Alternative Names
Arylamine N-acetyltransferase 1;2.3.1.5;Arylamide acetylase 1;Monomorphic arylamine N-acetyltransferase;MNAT;N-acetyltransferase type 1;NAT-1;NAT1;AAC1; NAT1 AAC1, MNAT, NAT-1, NATI N-acetyltransferase 1 arylamine N-acetyltransferase 1|N-acetyltransferase 1 (arylamine N-acetyltransferase)|N-acetyltransferase type 1|arylamide acetylase 1|monomorphic arylamine N-acetyltransferase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NAT1, check out the NAT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NAT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NAT1 Antibody Picoband® (A01386-1)
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Customer Q&As
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6 Customer Q&As for Anti-NAT1 Antibody Picoband®
Question
Does anti-NAT1 antibody A01386-1 work for WB with bronchial epithelial cell?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
According to the expression profile of bronchial epithelial cell, NAT1 is highly expressed in bronchial epithelial cell. So, it is likely that anti-NAT1 antibody A01386-1 will work for WB with bronchial epithelial cell.
Boster Scientific Support
Answered: 2019-10-28
Question
We are currently using anti-NAT1 antibody A01386-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
B. Moore
Verified customer
Asked: 2017-09-21
Answer
The anti-NAT1 antibody (A01386-1) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-21
Question
I was wanting to use your anti-NAT1 antibody for WB for human bronchial epithelial cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human bronchial epithelial cell identification?
K. Anderson
Verified customer
Asked: 2014-12-10
Answer
It shows on the product datasheet, A01386-1 anti-NAT1 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human bronchial epithelial cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-12-10
Question
Our lab want to know about to test anti-NAT1 antibody A01386-1 on human bronchial epithelial cell for research purposes, then I may be interested in using anti-NAT1 antibody A01386-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Singh
Verified customer
Asked: 2014-06-18
Answer
The products we sell, including anti-NAT1 antibody A01386-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-06-18
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bronchial epithelial cell using anti-NAT1 antibody A01386-1. Let me know if you need anything else.
V. Patel
Verified customer
Asked: 2014-03-17
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-03-17
Question
Do you have a BSA free version of anti-NAT1 antibody A01386-1 available?
G. Johnson
Verified customer
Asked: 2013-04-19
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NAT1 antibody A01386-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-04-19
