Product Info Summary
SKU: | A10707 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-PRIC285 HELZ2 Antibody
SKU/Catalog Number
A10707
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-PRIC285 HELZ2 Antibody (Catalog # A10707). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PRIC285 HELZ2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10707)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human PRIC285.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10707 is reactive to HELZ2 in Human
Reconstitution
Calculated molecular weight
294651 MW
Background of HELZ2
Helicase that acts as a transcriptional coactivator for a number of nuclear receptors including PPARA, PPARG, THRA, THRB and RXRA.
Surapureddi S., Proc. Natl. Acad. Sci. U.S.A. 99:11836-11841(2002).
Tomaru T., Endocrinology 147:377-388(2006).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10707 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from Jurkat cells, using PRIC285 antibody A10707.
Protein Target Info & Infographic
Gene/Protein Information For HELZ2 (Source: Uniprot.org, NCBI)
Gene Name
HELZ2
Full Name
Helicase with zinc finger domain 2
Weight
294651 MW
Superfamily
DNA2/NAM7 helicase family
Alternative Names
Helicase with zinc finger domain 2;ATP-dependent helicase PRIC285;Helicase with zinc finger 2, transcriptional coactivator;PPAR-alpha-interacting complex protein 285;PPAR-gamma DNA-binding domain-interacting protein 1;PDIP1;PPAR-gamma DBD-interacting protein 1;Peroxisomal proliferator-activated receptor A-interacting complex 285 kDa protein;3.6.4.-;HELZ2;KIAA1769, PRIC285; HELZ2 PDIP-1, PRIC285 helicase with zinc finger 2 helicase with zinc finger domain 2|ATP-dependent helicase PRIC285|PDIP1|PPAR gamma DBD-interacting protein 1|PPAR-alpha-interacting complex protein 285|PPAR-gamma DNA-binding domain-interacting protein 1|PPARG-DBD-interacting protein 1|PPARgamma-DNA-binding domain-interacting protein1|PRIC complex|helicase with zinc finger 2, transcriptional coactivator|peroxisomal proliferator-activated receptor A interacting complex 285|peroxisomal proliferator-activated receptor A-interacting complex 285 kDa protein|peroxisomal proliferator-activated receptor alpha-interacting cofactor complex, 285 kD subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HELZ2, check out the HELZ2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HELZ2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PRIC285 HELZ2 Antibody (A10707)
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No publications found for A10707
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-PRIC285 HELZ2 Antibody
Question
Is this A10707 anti-PRIC285 antibody reactive to the isotypes of HELZ2?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
The immunogen of A10707 anti-PRIC285 antibody is Synthesized peptide derived from internal of human PRIC285. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-19
Question
I was wanting to use to test anti-PRIC285 antibody A10707 on human cervix carcinoma for research purposes, then I may be interested in using anti-PRIC285 antibody A10707 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
The products we sell, including anti-PRIC285 antibody A10707, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-04
Question
We are currently using anti-PRIC285 antibody A10707 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
The anti-PRIC285 antibody (A10707) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-27
Question
I have a question about product A10707, anti-PRIC285 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
C. Gonzalez
Verified customer
Asked: 2019-06-21
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10707 anti-PRIC285 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-21
Question
Will A10707 anti-PRIC285 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-06-30
Answer
It shows on the product datasheet, A10707 anti-PRIC285 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-06-30
Question
Is a blocking peptide available for product anti-PRIC285 antibody (A10707)?
M. Krishna
Verified customer
Asked: 2016-01-14
Answer
We do provide the blocking peptide for product anti-PRIC285 antibody (A10707). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-01-14