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Product Info Summary
| SKU: | A00878-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-DYRK1A Antibody Picoband®
SKU/Catalog Number
A00878-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-DYRK1A Antibody Picoband® catalog # A00878-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DYRK1A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00878-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human DYRK1A recombinant protein (Position: H38-M269).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00878-1 is reactive to DYRK1A in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
100 kDa
Calculated molecular weight
85.584kDa
Background of DYRK1A
Dual specificity tyrosine-phosphorylation-regulated kinase 1A is an enzyme that in humans is encoded by the DYRK1A gene. This gene encodes a member of the Dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. It catalyzes its autophosphorylation on serine/threonine and tyrosine residues. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. This gene is a homolog of Drosophila mnb (minibrain) gene and rat Dyrk gene. It is localized in the Down syndrome critical region of chromosome 21, and is considered to be a strong candidate gene for learning defects associated with Down syndrome. Alternative splicing of this gene generates several transcript variants differing from each other either in the 5' UTR or in the 3' coding region. These variants encode at least five different isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00878-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence, 5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human Hela whole cell, rat skeletal muscle tissue
ICC/IF: U2OS cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of DYRK1A using anti-DYRK1A antibody (A00878-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: rat skeletal muscle tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DYRK1A antigen affinity purified polyclonal antibody (Catalog # A00878-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DYRK1A at approximately 100 kDa. The expected band size for DYRK1A is at 86 kDa.
Click image to see more details
Figure 2. IF analysis of DYRK1A using anti-DYRK1A antibody (A00878-1).
DYRK1A was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DYRK1A Antibody (A00878-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For DYRK1A (Source: Uniprot.org, NCBI)
Gene Name
DYRK1A
Full Name
Dual specificity tyrosine-phosphorylation-regulated kinase 1A
Weight
85.584kDa
Superfamily
protein kinase superfamily
Alternative Names
Dual specificity tyrosine-phosphorylation-regulated kinase 1A DYRK1A DYRK, DYRK1, HP86, MNB, MNBH, MRD7 dual specificity tyrosine phosphorylation regulated kinase 1A dual specificity tyrosine-phosphorylation-regulated kinase 1A|MNB/DYRK protein kinase|dual specificity YAK1-related kinase|dual specificity tyrosine-(Y)-phosphorylation regulated kinase 1A|mnb protein kinase homolog hp86|protein kinase minibrain homolog|serine/threonine kinase MNB|serine/threonine-specific protein kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DYRK1A, check out the DYRK1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DYRK1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DYRK1A Antibody Picoband® (A00878-1)
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Customer Q&As
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16 Customer Q&As for Anti-DYRK1A Antibody Picoband®
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for promyelocytic leukemia using anti-DYRK1A antibody A00878-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-13
Question
We have observed staining in mouse erythroleukemia. Any tips? Is anti-DYRK1A antibody supposed to stain erythroleukemia positively?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
According to literature erythroleukemia does express DYRK1A. According to Uniprot.org, DYRK1A is expressed in forebrain, fetal brain, promyelocytic leukemia, cervix carcinoma, leukemic t-cell, erythroleukemia, liver, among other tissues. Regarding which tissues have DYRK1A expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Erythroleukemia, Pubmed ID: 23186163
Fetal brain, Pubmed ID: 8769099, 8975710, 9503011
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Promyelocytic leukemia, Pubmed ID: 9037601
Boster Scientific Support
Answered: 2020-01-28
Question
I am interested in to test anti-DYRK1A antibody A00878-1 on rat promyelocytic leukemia for research purposes, then I may be interested in using anti-DYRK1A antibody A00878-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
The products we sell, including anti-DYRK1A antibody A00878-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-21
Question
I see that the anti-DYRK1A antibody A00878-1 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-07
Question
Do you have a BSA free version of anti-DYRK1A antibody A00878-1 available?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-DYRK1A antibody A00878-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-11
Question
Does anti-DYRK1A antibody A00878-1 work for Flow Cytometry with promyelocytic leukemia?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
According to the expression profile of promyelocytic leukemia, DYRK1A is highly expressed in promyelocytic leukemia. So, it is likely that anti-DYRK1A antibody A00878-1 will work for Flow Cytometry with promyelocytic leukemia.
Boster Scientific Support
Answered: 2019-09-10
Question
My colleagues were happy with the WB result of your anti-DYRK1A antibody. However we have been able to see positive staining in cervix carcinoma nucleus using this antibody. Is that expected? Could you tell me where is DYRK1A supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
According to literature, cervix carcinoma does express DYRK1A. Generally DYRK1A expresses in nucleus. Regarding which tissues have DYRK1A expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Erythroleukemia, Pubmed ID: 23186163
Fetal brain, Pubmed ID: 8769099, 8975710, 9503011
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Promyelocytic leukemia, Pubmed ID: 9037601
Boster Scientific Support
Answered: 2019-08-15
Question
I was wanting to use your anti-DYRK1A antibody for Flow Cytometry for rat promyelocytic leukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat promyelocytic leukemia identification?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
As indicated on the product datasheet, A00878-1 anti-DYRK1A antibody has been tested for ELISA, Flow Cytometry, IF, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat promyelocytic leukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-03
Question
Will A00878-1 anti-DYRK1A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-04-08
Answer
It shows on the product datasheet, A00878-1 anti-DYRK1A antibody as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-04-08
Question
I have a question about product A00878-1, anti-DYRK1A antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-03-08
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00878-1 anti-DYRK1A antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-03-08
Question
Our lab want to know about using your anti-DYRK1A antibody for protein phosphorylation studies. Has this antibody been tested with western blotting on siha cells? We would like to see some validation images before ordering.
G. Rodriguez
Verified customer
Asked: 2019-02-18
Answer
Thanks for your inquiry. This A00878-1 anti-DYRK1A antibody is tested on human hela, hela whole cell lysates, rat skeletal muscle tissue, mouse skeletal muscle tissue, siha cells. It is guaranteed to work for ELISA, Flow Cytometry, IF, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-02-18
Question
We are currently using anti-DYRK1A antibody A00878-1 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-05-23
Answer
The anti-DYRK1A antibody (A00878-1) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-23
Question
We ordered your anti-DYRK1A antibody for WB on liver last year. I am using human, and We intend to use the antibody for ELISA next. I would like examining liver as well as leukemic t-cell in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?
R. Li
Verified customer
Asked: 2018-02-14
Answer
I looked at the website and datasheets of our anti-DYRK1A antibody and it seems that A00878-1 has been validated on human in both WB and ELISA. Thus A00878-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-02-14
Question
See below the WB image, lot number and protocol we used for promyelocytic leukemia using anti-DYRK1A antibody A00878-1. Please let me know if you require anything else.
L. Johnson
Verified customer
Asked: 2017-11-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-16
Question
Is a blocking peptide available for product anti-DYRK1A antibody (A00878-1)?
J. Kulkarni
Verified customer
Asked: 2016-06-01
Answer
We do provide the blocking peptide for product anti-DYRK1A antibody (A00878-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-06-01
Question
Is this A00878-1 anti-DYRK1A antibody reactive to the isotypes of DYRK1A?
R. Patel
Verified customer
Asked: 2015-01-16
Answer
The immunogen of A00878-1 anti-DYRK1A antibody is E. coli-derived human DYRK1A recombinant protein (Position: H38-M269). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-01-16
