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- Table of Contents
Facts about Prolactin regulatory element-binding protein.
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Human | |
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Gene Name: | PREB |
Uniprot: | Q9HCU5 |
Entrez: | 10113 |
Belongs to: |
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No superfamily |
MGC3467; PREB; prolactin regulatory binding-element protein; prolactin regulatory element binding; prolactin regulatory element-binding protein; SEC12; SEC12Mammalian guanine nucleotide exchange factor mSec12
Mass (kDA):
45.468 kDA
Human | |
---|---|
Location: | 2p23.3 |
Sequence: | 2; NC_000002.12 (27130756..27134675, complement) |
Endoplasmic reticulum membrane; Single-pass membrane protein. Nucleus. Concentrates at endoplasmic reticulum exit sites (ERES), also known as transitional endoplasmic reticulum (tER).
Boster Bio is a good place to start your research project if you're looking for a reliable anti-body. Boster Bio was founded in 1993. Their signature products include ELISA kit and antibodies. They started providing PCR-related products in molecular Biology more recently. As an added bonus, they offer free technical resources and 24 hour support. Continue reading to discover why Boster is a trusted brand for researchers.
The high affinity primary antibodies of Boster are highly regarded by the research community. These antibodies are validated on immunohistochemistry, Western Blotting, and ELISA protocols. In addition, Boster's reagents are highly cited in scientific journals. Researchers will find it easy to locate and purchase the right reagents through detailed product citations.
These high-quality antibodies include ANTI–MOUSE/HUMAN MAC-2(GALECTIN-33) antibody, ANTI–Human IgA and Rabbit Anti–GapDH. Boster offers a wide array of monoclonal antibodies, including conjugated, unconjugated and biotinylated.
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Steven Boster was the inventor of his first product. His company became the largest catalog-based antibody company in China by the end if the decade. He patented hundreds upon hundreds of primary antibodies, and he also developed PicoKine(tm), his own proprietary ELISA platform. He then used the proprietary trade secrets to create high-sensitivity ELISA kits. Boster was an invited speaker at many scientific conferences after that and was well-known for sharing his infectious enthusiasm.
Secondary antibodies are useful for cross-reactivity detection. However, they don't necessarily reflect the quality or purity of the primary antibody. Secondary antibodies may not bind to the same epitopes as the primary antibody. Secondary antibodies should not be used as controls, if the primary antibody does cross-react. Dual antibodies require greater optimization but are more sensitive.
To ensure that Boster’s primary antibodies work, they are tested against multiple types of protein and tissue. Ambroz et al. (62) validated the binding of the antibody against MRP1. Ab233383 displays a glycosylated antibody against MRP1. Odyssey CLx imager has 169 mm resolution and auto-intensity.
In addition to ensuring antibody specificity and sensitivity, Boster's primary antibodies are also rigorously validated on Immunohistochemistry, Western Blotting, and ELISA. Immunohistochemistry (IHC), which uses tissue sections to demonstrate protein expression in context, uses immunohistochemistry (IHC). You can detect the results of ELISA either directly or indirectly. Direct ELISA assays label antibodies using alkaline phosphatase. Indirect ELISA Assays use HRP substrates.
The specificity and selectivity are key factors in the performance of primary antibodies used in these assays. There are different guidelines to validate antibodies. The assay context is vital because even small variations in the assay conditions could affect the performance. It is essential to ensure the highest quality of primary antibodies and for reproducible results that the reagents are validated.
Validating ELISA and immunehistochemistry validation can prove difficult. But Boster has rigorously validated its primary antibodies for reproducible results. For immunohistochemistry, this means that they are tested for specificity using additional antigens, such as trichrome-labeled proteins or cell membranes. It doesn't end there!
Different experimental approaches necessitate different antibody properties. For example, western Blotting may reveal a linear sequence of amino-acids that has been denaturated SDS/PAGE. It may be more or less natural in immunoisolation or immunoprecipitation. Cross-linking of the antigen can be used to identify tissue or cell samples. In each case, the antibody must be able to detect the cross-linked structures in the target sample. These applications require antibody specificity, but it is not a guarantee for reproducibility.
Scientists and manufacturers can reap many benefits from recombinant monoclonal antibodies. Recombinant antibody are more specific than traditional monoclonal antibodies and can be produced in higher volumes in a shorter time. They are also more consistent and have unlimited supply. You will not need additional antibodies if multiple copies are required.
Collaboration is key to reproducibility. This collaboration includes the efforts of the publisher, antibody vendor, as well as the user. To ensure reproducibility, the user should validate and design experiments that will verify the performance of the antibody. The vendor should supply high-quality antibodies as well as detailed disclosure of methods. The publisher should be able to enforce standards for data reporting. Additionally, the antibody vendor should provide feedback about the antibodies it sells.
PMID: 10920239 by Taylor Clelland C.L., et al. Cloning and characterization of human PREB; a gene that maps to a genomic region associated with trisomy 2p syndrome.
PMID: 12735795 by Edgar A.J.; The gene structure and expression of human ABHD1: overlapping polyadenylation signal sequence with Sec12.