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Product Info Summary
| SKU: | A09894-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-PPP2R5B Antibody (C-term)
SKU/Catalog Number
A09894-1
Size
80 µl
Description
Boster Bio Anti-PPP2R5B Antibody (C-term) (Catalog # A09894-1). Tested in IF, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PPP2R5B Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09894-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This PPP2R5B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 466-500 amino acids from the C-terminal region of human PPP2R5B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09894-1 is reactive to PPP2R5B in Human
Reconstitution
Calculated molecular weight
57393 Da
Background of PPP2R5B
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The phosphorylated form mediates the interaction between AKT1 and PP2A phosphatase leading to dephosphorylation of AKT1 on the 'Thr-308' and 'Ser-373' residues.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09894-1 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:1000
Validation Images & Assay Conditions
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Fluorescent image of SH-SY5Y cells stained with PPP2R5B Antibody (C-term). A09894-1 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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Western blot analysis of lysate from SK-N-MC cell line, using PPP2R5B Antibody (C-term) (Cat. #A09894-1). A09894-1 was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Protein Target Info & Infographic
Gene/Protein Information For PPP2R5B (Source: Uniprot.org, NCBI)
Gene Name
PPP2R5B
Full Name
Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform
Weight
57393 Da
Superfamily
phosphatase 2A regulatory subunit B56 family
Alternative Names
Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform, PP2A B subunit isoform B'-beta, PP2A B subunit isoform B56-beta, PP2A B subunit isoform PR61-beta, PP2A B subunit isoform R5-beta, PPP2R5B PPP2R5B B56B, B56beta, PR61B protein phosphatase 2 regulatory subunit Bbeta serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform|protein phosphatase 2 regulatory subunit B, beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPP2R5B, check out the PPP2R5B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPP2R5B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PPP2R5B Antibody (C-term) (A09894-1)
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