Product Info Summary
SKU: | A05935 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ICC, WB |
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Product info
Product Name
Anti-PPP2R5A Antibody
SKU/Catalog Number
A05935
Size
100ul
Form
Liquid
Description
Boster Bio Anti-PPP2R5A Antibody catalog # A05935. Tested in WB,ICC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PPP2R5A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05935)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human S26A5
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05935 is reactive to PPP2R5A in Human, Mouse, Rat
Applications
A05935 is guaranteed for ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
56194 MW
Background of PPP2R5A
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Required for the integrity and for normal function of the Golgi apparatus and the trans-Golgi network. Plays a role in insulin-stimulated translocation of GLUT4 to the cell membrane. Plays a role in M6PR transport from the trans-Golgi network to endosomes. Plays a role in the internalization of EGFR from the cell membrane into endosomes. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis.
Chen D., Gene 174:129-134(1996) [PubMed: 8863739].
Bao X.,Eur. J. Biochem. 269:259-271(2002) [PubMed: 11784320].
Ota T., Nat. Genet. 36:40-45(2004) [PubMed: 14702039].<
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
ICC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-PPP2R5A Antibody A05935
Western blot (WB) analysis of PPP2R5A polyclonal antibody at 1:500 dilutionLane1:Hela cell lysate
Lane2:NIH-3T3 cell lysate
Lane3:H9C2 cell lysate
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For PPP2R5A (Source: Uniprot.org, NCBI)
Gene Name
PPP2R5A
Full Name
Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform
Weight
56194 MW
Superfamily
phosphatase 2A regulatory subunit B56 family
Alternative Names
B56A; MGC131915; PP2A B subunit isoform B56-alpha; PP2A B subunit isoform B'-alpha; PP2A B subunit isoform PR61-alpha; PP2A B subunit isoform R5-alpha; PP2A, B subunit, B' alpha isoform; PP2A, B subunit, B56 alpha isoform; PP2A, B subunit, PR61 alpha isoform; PP2A, B subunit, R5 alpha isoform; PR61A; PR61alpha; protein phosphatase 2, regulatory subunit B (B56), alpha isoform; protein phosphatase 2, regulatory subunit B', alpha isoform; protein phosphatase 2, regulatory subunit B', alpha; serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, alphaisoform; serine/threonine-protein PPP2R5A B56A, B56alpha, PR61A protein phosphatase 2 regulatory subunit Balpha serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform|PR61alpha|protein phosphatase 2 regulatory subunit B, alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPP2R5A, check out the PPP2R5A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPP2R5A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PPP2R5A Antibody (A05935)
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