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Product Info Summary
| SKU: | A09697 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-PPP1R3B Antibody Picoband®
SKU/Catalog Number
A09697
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PPP1R3B Antibody Picoband® catalog # A09697. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PPP1R3B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09697)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PPP1R3B recombinant protein (Position: M1-Y285).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A09697 is reactive to PPP1R3B in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
33 kDa
Calculated molecular weight
32.695kDa
Background of PPP1R3B
This gene encodes the catalytic subunit of the serine/theonine phosphatase, protein phosphatase-1. The encoded protein is expressed in liver and skeletal muscle tissue and may be involved in regulating glycogen synthesis in these tissues. This gene may be a involved in type 2 diabetes and maturity-onset diabetes of the young. Alternate splicing results in multiple transcript variants that encode the same protein.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09697 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human hepatocellular carcinoma tumor tissue (HCCT), human hepatocellular carcinoma paracancerous tissue (HCCP)
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PPP1R3B using anti-PPP1R3B antibody (A09697).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates,
Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1R3B antigen affinity purified polyclonal antibody (Catalog # A09697) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP1R3B at approximately 33 kDa. The expected band size for PPP1R3B is at 33 kDa.
Protein Target Info & Infographic
Gene/Protein Information For PPP1R3B (Source: Uniprot.org, NCBI)
Gene Name
PPP1R3B
Full Name
Protein phosphatase 1 regulatory subunit 3B
Weight
32.695kDa
Alternative Names
One cut domain family member 2; Hepatocyte nuclear factor 6-beta; HNF-6-beta; One cut homeobox 2; Transcription factor ONECUT-2; OC-2; ONECUT2; HNF6B PPP1R3B GL, PPP1R4, PTG protein phosphatase 1 regulatory subunit 3B protein phosphatase 1 regulatory subunit 3B|PP1 subunit R4|hepatic glycogen-targeting protein phosphatase 1 regulatory subunit GL|hepatic glycogen-targeting subunit, G(L)|protein phosphatase 1 regulatory subunit 4|protein phosphatase 1, regulatory (inhibitor) subunit 3B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPP1R3B, check out the PPP1R3B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPP1R3B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PPP1R3B Antibody Picoband® (A09697)
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