Anti-PPP1R3B Antibody Picoband™

Product Name

Anti-PPP1R3B Antibody Picoband™

View all PPP1R3B Antibodies

SKU/Catalog Number

A09697

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-PPP1R3B Antibody Picoband™ catalog # A09697. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PPP1R3B Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09697)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PPP1R3B recombinant protein (Position: M1-Y285).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A09697 is reactive to PPP1R3B in Human

Applications

A09697 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

33 kDa

Calculated molecular weight

Background of PPP1R3B

This gene encodes the catalytic subunit of the serine/theonine phosphatase, protein phosphatase-1. The encoded protein is expressed in liver and skeletal muscle tissue and may be involved in regulating glycogen synthesis in these tissues. This gene may be a involved in type 2 diabetes and maturity-onset diabetes of the young. Alternate splicing results in multiple transcript variants that encode the same protein.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PPP1R3B using anti-PPP1R3B antibody (A09697).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates,
Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1R3B antigen affinity purified polyclonal antibody (Catalog # A09697) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP1R3B at approximately 33 kDa. The expected band size for PPP1R3B is at 33 kDa.

Gene/Protein Information For PPP1R3B (Source: Uniprot.org, NCBI)

Alternative Names

FLJ14005; FLJ34675; GL; Hepatic glycogen-targeting protein phosphatase 1 regulatory subunit GL; PPP1R4PP1 subunit R4; protein phosphatase 1 regulatory subunit 3B; Protein phosphatase 1 regulatory subunit 4; Protein phosphatase 1 subunit GL; protein phosphatase 1, regulatory (inhibitor) subunit 3B PPP1R3B GL, PPP1R4, PTG protein phosphatase 1 regulatory subunit 3B protein phosphatase 1 regulatory subunit 3B|PP1 subunit R4|hepatic glycogen-targeting protein phosphatase 1 regulatory subunit GL|hepatic glycogen-targeting subunit, G(L)|protein phosphatase 1 regulatory subunit 4|protein phosphatase 1, regulatory (inhibitor) subunit 3B

For more info on PPP1R3B, check out the PPP1R3B Infographic

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In this infographic, you will see the following information for PPP1R3B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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