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Product Info Summary
| SKU: | A08303 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-NETO1 Antibody
SKU/Catalog Number
A08303
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-NETO1 Antibody (Catalog # A08303). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
NETO1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-NETO1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08303)
Host
Rabbit
Contents
NETO1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
NETO1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human NETO1. The immunogen is located within amino acids 350 - 400 of NETO1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
NETO1 antibody is predicted to not cross-react with other NETO protein family members.
Reactive Species
A08303 is reactive to NETO1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
60191 MW
Background of NETO1
Neuropilin and tolloid-like protein 1 (NETO1) is involved in the development and/or maintenance of neuronal circuitry. It is a type I transmembrane protein that is expressed in the brain and retina. NETO1 contains one LDL-receptor class A domain and two CUB domains and is either membrane-bound or secreted. It has three alternatively spliced isoforms, the first two of which are retina-specific and the third of which is found in both retina and brain tissue. Furthermore, as an accessory subunit of the neuronal N-methyl-D-aspartate receptor (NMDAR), it regulates long-term NMDA receptor-dependent synaptic plasticity and cognition, at least in the context of spatial learning and memory.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08303 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
NETO1 antibody can be used for detection of NETO1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of NETO1 in human brain tissue lysate with NETO1 antibody at 0.25 μg/mL.
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Immunohistochemistry of NETO1 in rat lung tissue with NETO1 antibody at 2.5 μg/mL.
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Immunofluorescence of NETO1 in rat lung tissue with NETO1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For NETO1 (Source: Uniprot.org, NCBI)
Gene Name
NETO1
Full Name
Neuropilin and tolloid-like protein 1
Weight
60191 MW
Alternative Names
BCTL1, BTCL1, Neuropilin and tolloid-like protein 1, Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1 NETO1 BCTL1, BTCL1 neuropilin and tolloid like 1 neuropilin and tolloid-like protein 1|brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1|neuropilin (NRP) and tolloid (TLL)-like 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NETO1, check out the NETO1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NETO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NETO1 Antibody (A08303)
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