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Product Info Summary
| SKU: | A14650-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-PPP1R16A Antibody Picoband®
SKU/Catalog Number
A14650-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PPP1R16A Antibody Picoband® catalog # A14650-1. Tested in WB, FCM, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PPP1R16A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14650-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human PPP1R16A recombinant protein (Position: R55-M528). Human PPP1R16A shares 81.2% amino acid (aa) sequence identity with mouse PPP1R16A.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A14650-1 is reactive to PPP1R16A in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
69 kDa
Calculated molecular weight
57.53kDa
Background of Ppp1r16a
PPP1R16A inhibits protein phosphatase 1 activity toward phosphorylase, myosin light chain and myosin substrates (Bysimilarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14650-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HepG2 whole cell, human MCF-7 whole cell, human PC-3 whole cell, human U20S whole cell
FCM: U20S cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PPP1R16A using anti-PPP1R16A antibody (A14650-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human U20S whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1R16A antigen affinity purified polyclonal antibody (Catalog # A14650-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP1R16A at approximately 69 kDa. The expected band size for PPP1R16A is at 58 kDa.
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Figure 2. Flow Cytometry analysis of U20S cells using anti-PPP1R16A antibody (A14650-1).
Overlay histogram showing U20S cells stained with A14650-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP1R16A Antibody (A14650-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For PPP1R16A (Source: Uniprot.org, NCBI)
Gene Name
PPP1R16A
Full Name
Protein phosphatase 1 regulatory subunit 16A
Weight
57.53kDa
Alternative Names
likley ortholog of mouse myosin phosphatase targeting subunit 3; Myosin phosphatase-targeting subunit 3; MYPT3MGC14333; protein phosphatase 1 regulatory subunit 16A; protein phosphatase 1, regulatory (inhibitor) subunit 16A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPP1R16A, check out the PPP1R16A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPP1R16A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PPP1R16A Antibody Picoband® (A14650-1)
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