Product Info Summary
SKU: | A12606 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-CHST13 Antibody
SKU/Catalog Number
A12606
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-CHST13 Antibody (Catalog # A12606). Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CHST13 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12606)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human CHST13.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12606 is reactive to CHST13 in Human
Applications
A12606 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
38920 MW
Background of CHST13
Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Transfers sulfate to the C4 hydroxyl of beta1,4-linked GalNAc that is substituted with a beta-linked glucuronic acid at the C-3 hydroxyl. No activity toward dermatan.
Kang H.-G., J. Biol. Chem. 277:34766-34772(2002).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
![a12606 western blotting analysis 1 a12606 western blotting analysis 1](https://www.bosterbio.com/media/catalog/product/a/1/a12606-western-blotting-analysis-1.jpg)
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Western blot analysis of extracts from K562 cells, using CHST13 antibody A12606.
![a12606 immunohistochemistry analysis 1 a12606 immunohistochemistry analysis 1](https://www.bosterbio.com/media/catalog/product/a/1/a12606-immunohistochemistry-analysis-1.jpg)
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Immunohistochemistry analysis of paraffin-embedded human testis tissue, using CHST13 antibody A12606.
Protein Target Info & Infographic
Gene/Protein Information For CHST13 (Source: Uniprot.org, NCBI)
Gene Name
CHST13
Full Name
Carbohydrate sulfotransferase 13
Weight
38920 MW
Superfamily
sulfotransferase 2 family
Alternative Names
C4ST-3; C4ST3MGC119279; carbohydrate (chondroitin 4) sulfotransferase 13; carbohydrate sulfotransferase 13; Chondroitin 4-O-sulfotransferase 3; Chondroitin 4-sulfotransferase 3; EC 2.8.2.5; MGC119278; MGC119281 CHST13 C4ST3 carbohydrate sulfotransferase 13 carbohydrate sulfotransferase 13|C4ST-3|carbohydrate (chondroitin 4) sulfotransferase 13|chondroitin 4-O-sulfotransferase 3|chondroitin 4-sulfotransferase 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CHST13, check out the CHST13 Infographic
![CHST13 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CHST13: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CHST13 Antibody (A12606)
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No publications found for A12606
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Customer Q&As
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3 Customer Q&As for Anti-CHST13 Antibody
Question
I was wanting to use your anti-CHST13 antibody for WB for human right lobe of liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human right lobe of liver identification?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
As indicated on the product datasheet, A12606 anti-CHST13 antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human right lobe of liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-31
Question
We are currently using anti-CHST13 antibody A12606 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
K. Jones
Verified customer
Asked: 2018-02-28
Answer
The anti-CHST13 antibody (A12606) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-28
Question
Would anti-CHST13 antibody A12606 work for WB with right lobe of liver?
R. Wu
Verified customer
Asked: 2017-09-20
Answer
According to the expression profile of right lobe of liver, CHST13 is highly expressed in right lobe of liver. So, it is likely that anti-CHST13 antibody A12606 will work for WB with right lobe of liver.
Boster Scientific Support
Answered: 2017-09-20