Click image to see more details
Product Info Summary
| SKU: | M02801 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-PPP1CA+PPP1CB Rabbit Monoclonal Antibody
SKU/Catalog Number
M02801
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PPP1CA+PPP1CB Rabbit Monoclonal Antibody catalog # M02801. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PPP1CA+PPP1CB Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02801)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFC-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PPP1CA + 1CB
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02801 is reactive to PPP1CA in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
38 kDa
Calculated molecular weight
37512 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02801 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: human Hela whole cell, human Jurkat whole cell, human K562 whole cell, human U87 whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse NIH/3T3 whole cell
IHC: human pancreas tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PPP1CA + PPP1CB using anti-PPP1CA + PPP1CB antibody (M02801).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human U87 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1CA + PPP1CB antigen affinity purified monoclonal antibody (Catalog # M02801) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP1CA + PPP1CB at approximately 38 kDa. The expected band size for PPP1CA + PPP1CB is at 38 kDa.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human pancreas, using PPP1CA+PPP1CB Antibody(M02801)
PPP1CA was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PPP1CA Antibody (M02801)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For PPP1CA (Source: Uniprot.org, NCBI)
Gene Name
PPP1CA
Full Name
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
Weight
37512 MW
Superfamily
PPP phosphatase family
Alternative Names
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit;PP-1A;3.1.3.16;PPP1CA;PPP1A; PPP1CA PP-1A, PP1A, PP1alpha, PPP1A protein phosphatase 1 catalytic subunit alpha serine/threonine-protein phosphatase PP1-alpha catalytic subunit|protein phosphatase 1, catalytic subunit, alpha isozyme|serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit|testicular tissue protein Li 155
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPP1CA, check out the PPP1CA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPP1CA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PPP1CA+PPP1CB Rabbit Monoclonal Antibody (M02801)
Hello CJ!
No publications found for M02801
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-PPP1CA+PPP1CB Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-PPP1CA+PPP1CB Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-PPP1CA+PPP1CB Rabbit Monoclonal Antibody
Question
I have attached the WB image, lot number and protocol we used for lung using anti-PPP1CA+PPP1CB Rabbit Monoclonal antibody M02801. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-17
Question
We are currently using anti-PPP1CA+PPP1CB Rabbit Monoclonal antibody M02801 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
The anti-PPP1CA+PPP1CB Rabbit Monoclonal antibody (M02801) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-31
Question
I was wanting to use your anti-PPP1CA+PPP1CB Rabbit Monoclonal antibody for IF for mouse lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse lung identification?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
You can see on the product datasheet, M02801 anti-PPP1CA+PPP1CB Rabbit Monoclonal antibody has been tested for IP, IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-28
Question
I have a question about product M02801, anti-PPP1CA+PPP1CB Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Miller
Verified customer
Asked: 2017-10-19
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02801 anti-PPP1CA+PPP1CB Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-10-19
Question
I was wanting to use to test anti-PPP1CA+PPP1CB Rabbit Monoclonal antibody M02801 on mouse lung for research purposes, then I may be interested in using anti-PPP1CA+PPP1CB Rabbit Monoclonal antibody M02801 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-10-10
Answer
The products we sell, including anti-PPP1CA+PPP1CB Rabbit Monoclonal antibody M02801, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-10
Question
Do you have a BSA free version of anti-PPP1CA+PPP1CB Rabbit Monoclonal antibody M02801 available?
F. Carter
Verified customer
Asked: 2015-01-15
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PPP1CA+PPP1CB Rabbit Monoclonal antibody M02801 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-01-15
