Anti-PPP1CA+PPP1CB Rabbit Monoclonal Antibody

PPP1CA antibody

Boster Bio Anti-PPP1CA+PPP1CB Rabbit Monoclonal Antibody catalog # M02801. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M02801
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IP, IF, IHC, ICC, WB

Product Name

Anti-PPP1CA+PPP1CB Rabbit Monoclonal Antibody

View all PPP1CA Antibodies

SKU/Catalog Number

M02801

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-PPP1CA+PPP1CB Rabbit Monoclonal Antibody catalog # M02801. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PPP1CA+PPP1CB Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02801)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AFC-16

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human PPP1CA + 1CB

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M02801 is reactive to PPP1CA in Human, Mouse, Rat

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

38 kDa

Calculated molecular weight

37512 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

M02801 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50

Positive Control

WB: human Hela whole cell, human Jurkat whole cell, human K562 whole cell, human U87 whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse NIH/3T3 whole cell
IHC: human pancreas tissue

Validation Images & Assay Conditions

Gene/Protein Information For PPP1CA (Source: Uniprot.org, NCBI)

Gene Name

PPP1CA

Full Name

Serine/threonine-protein phosphatase PP1-alpha catalytic subunit

Weight

37512 MW

Superfamily

PPP phosphatase family

Alternative Names

Serine/threonine-protein phosphatase PP1-alpha catalytic subunit;PP-1A;3.1.3.16;PPP1CA;PPP1A; PPP1CA PP-1A, PP1A, PP1alpha, PPP1A protein phosphatase 1 catalytic subunit alpha serine/threonine-protein phosphatase PP1-alpha catalytic subunit|protein phosphatase 1, catalytic subunit, alpha isozyme|serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit|testicular tissue protein Li 155

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PPP1CA, check out the PPP1CA Infographic

PPP1CA infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PPP1CA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-PPP1CA+PPP1CB Rabbit Monoclonal Antibody

Question

I have attached the WB image, lot number and protocol we used for lung using anti-PPP1CA+PPP1CB Rabbit Monoclonal antibody M02801. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-03-17

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-17

Question

We are currently using anti-PPP1CA+PPP1CB Rabbit Monoclonal antibody M02801 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-31

Answer

The anti-PPP1CA+PPP1CB Rabbit Monoclonal antibody (M02801) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-31

Question

I was wanting to use your anti-PPP1CA+PPP1CB Rabbit Monoclonal antibody for IF for mouse lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse lung identification?

Verified Customer

Verified customer

Asked: 2019-10-28

Answer

You can see on the product datasheet, M02801 anti-PPP1CA+PPP1CB Rabbit Monoclonal antibody has been tested for IP, IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse lung in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-28

Question

I have a question about product M02801, anti-PPP1CA+PPP1CB Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

B. Miller

Verified customer

Asked: 2017-10-19

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02801 anti-PPP1CA+PPP1CB Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-10-19

Question

I was wanting to use to test anti-PPP1CA+PPP1CB Rabbit Monoclonal antibody M02801 on mouse lung for research purposes, then I may be interested in using anti-PPP1CA+PPP1CB Rabbit Monoclonal antibody M02801 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-10-10

Answer

The products we sell, including anti-PPP1CA+PPP1CB Rabbit Monoclonal antibody M02801, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-10-10

Question

Do you have a BSA free version of anti-PPP1CA+PPP1CB Rabbit Monoclonal antibody M02801 available?

F. Carter

Verified customer

Asked: 2015-01-15

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PPP1CA+PPP1CB Rabbit Monoclonal antibody M02801 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2015-01-15

Order DetailsPrice
M02801

100μL

$370
M02801-30ul

30μL sample

$99
M02801-carrier-free-1ml

Carrier Free

$2590

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M02801
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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