Human PP1 Catalytic Recombinant Protein

PPP1CA protein,

Human PP1 Catalytic Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-330aa. Application(s): SDS-PAGE, Enzyme Activity.

Product Info Summary

SKU: PROTP62136
Size: Starting from 10ug
Source: E. coli
Application: Enzyme Activity, SDS-PAGE

Product Name

Human PP1 Catalytic Recombinant Protein

View all PPP1CA recombinant proteins

SKU/Catalog Number

PROTP62136

Size

Starting from 10ug

Tag

His-Tag

Description

Human PP1 Catalytic Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-330aa. Application(s): SDS-PAGE, Enzyme Activity.

Storage & Handling

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)

Cite This Product

Human PP1 Catalytic Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP62136)

Form

Liquid

Formulation

50mM Tris-HCl buffer (pH 8.5) containing 0.2M NaCl, 1mM DTT, 0.1mM PMSF, 1mM MnCl2, 50%glycerol

Concentration

0.2mg/ml (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Predicted MW

39.7 kDa (350aa) confirmed by MALDI-TOF

Amino Acid Sequence

Human, P62136, 1-330aa; MSDSEKLNLD SIIGRLLEVQ GSRPGKNVQL TENEIRGLCL KSREIFLSQP ILLELEAPLK ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS LETICLLLAY KIKYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL LCDLLWSDPD KDVQGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQV VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAD KNKGKYGQFS GLNPGGRPIT PPRNSAKAKK

Bioactivity

Specific activity is >3,000 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37°C, pH7.5 using 10mM of substrate.

Validation Images & Assay Conditions

Gene/Protein Information For PPP1CA (Source: Uniprot.org, NCBI)

Gene Name

PPP1CA

Full Name

Serine/threonine-protein phosphatase PP1-alpha catalytic subunit

Weight

39.7 kDa (350aa) confirmed by MALDI-TOF

Superfamily

PPP phosphatase family

Alternative Names

Serine/threonine-protein phosphatase PP1-alpha catalytic subunit, PP-1A, PP1alpha, PPP1A PPP1CA PP-1A, PP1A, PP1alpha, PPP1A protein phosphatase 1 catalytic subunit alpha serine/threonine-protein phosphatase PP1-alpha catalytic subunit|protein phosphatase 1, catalytic subunit, alpha isozyme|serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit|testicular tissue protein Li 155

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on PPP1CA, check out the PPP1CA Infographic

PPP1CA infographic

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In this infographic, you will see the following information for PPP1CA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].

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Size

Total: $350

SKU:PROTP62136

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