Anti-PPME1 Mouse Monoclonal Antibody [Clone ID: OTI7F12]

Product Name

Anti-PPME1 Mouse Monoclonal Antibody [Clone ID: OTI7F12]

View all PPME1 Antibodies

SKU/Catalog Number

M08083

Size

100 µl

Description

Boster Bio Anti-PPME1 mouse monoclonal antibody, clone OTI7F12 (formerly 7F12). Catalog# M08083. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C as received.

Cite This Product

Anti-PPME1 Mouse Monoclonal Antibody [Clone ID: OTI7F12] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08083)

Host

Mouse

Contents

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Clonality

Monoclonal

Clone Number

OTI7F12

Isotype

IgG1

Immunogen

Full length human recombinant protein of human PPME1 (NP_057231) produced in HEK293T cell.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M08083 is reactive to PPME1 in Human, Mouse, Rat

Applications

M08083 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Validation Images & Assay Conditions

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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPME1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPME1.

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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPME1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-PPME1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

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Flow cytometric analysis of Hela cells

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Flow cytometric analysis of Jurkat cells

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Anti-PPME1 mouse monoclonal antibody (M08083) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPME1.

Gene/Protein Information For PPME1 (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.1.1; EC 3.1.1.-; FLJ22226; PME-1PME1; protein phosphatase methylesterase 1; protein phosphatase methylesterase-1 PPME1 ABDH19, PME-1 protein phosphatase methylesterase 1 protein phosphatase methylesterase 1|testicular secretory protein Li 39

For more info on PPME1, check out the PPME1 Infographic

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In this infographic, you will see the following information for PPME1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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