Anti-PPIF Antibody

Product Name

Anti-PPIF Antibody

View all Cyclophilin-F Antibodies

SKU/Catalog Number

A02803

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-PPIF Antibody (Catalog # A02803). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PPIF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02803)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human PPIF.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02803 is reactive to PPIF in Human, Mouse, Rat

Reconstitution

Calculated molecular weight

22040 MW

Background of Cyclophilin-F

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Involved in regulation of the mitochondrial permeability transition pore (mPTP). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probablity of the mPTP leading to apoptosis or necrosis; the requirement of the PPIase activity for this function is debated. In cooperation with mitochondrial TP53 is involved in activating oxidative stress-induced necrosis. Involved in modulation of mitochondrial membrane F1F0 ATP synthase activity and regulation of mitochondrial matrix adenine nucleotide levels. Has anti-apoptotic activity independently of mPTP and in cooperation with BCL2 inhibits cytochrome c-dependent apoptosis.

Bergsma D.J., J. Biol. Chem. 266:23204-23214(1991).
Deloukas P., Nature 429:375-381(2004).
The MGC Project Team, Genome Res. 14:2121-2127(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A02803 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Validation Images & Assay Conditions

Gene/Protein Information For PPIF (Source: Uniprot.org, NCBI)

Alternative Names

Peptidyl-prolyl cis-trans isomerase F, mitochondrial;PPIase F;5.2.1.8;Cyclophilin D;CyP-D;CypD;Cyclophilin F;Mitochondrial cyclophilin;CyP-M;Rotamase F;PPIF;CYP3; PPIF CYP3, CyP-M, Cyp-D, CypD peptidylprolyl isomerase F peptidyl-prolyl cis-trans isomerase F, mitochondrial|PPIase F|cyclophilin 3|cyclophilin D|cyclophilin F|mitochondrial cyclophilin|peptidyl-prolyl cis-trans isomerase, mitochondrial|rotamase F

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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