PPIF Antibodies

Peptidyl-prolyl cis-trans isomerase F, mitochondrial (PPIF)

PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (PubMed:20676357). Involved in regulation of the mitochondrial permeability transition pore (mPTP).

It is suggested that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis; the requirement of the PPIase activity for this particular purpose is debated. In cooperation with mitochondrial TP53 is involved in activating oxidative stress-induced necrosis.

Gene Information

Human
Gene Name:	PPIF
Uniprot:	P30405
Entrez:	10105

Family

Belongs to:
cyclophilin-type PPIase family

Alternative Names

Cyclophilin F; CYP3FLJ90798; Cyp-D; hCyP3; MGC117207; mitochondrial; peptidyl-prolyl cis-trans isomerase, mitochondrial; peptidylprolyl isomerase F (cyclophilin F); peptidylprolyl isomerase F

Molecular Weight

Mass (kDA):
22.04 kDA

Genomic Context

Human
Location:	10q22.3
Sequence:	10; NC_000010.11 (79347469..79355334)

Subcellular Localizations

Mitochondrion matrix.

Diseases

• Edema
• Cardiomyopathies
• Cardiomyopathy, Dilated
• Heart Failure
• Cardiomyopathy, Familial Idiopathic
• Abnormal Behavior
• Right Ventricular Hypertrophy
• Malnutrition
• Drug-induced Liver Injury
• Cardiac Hypertrophy
• Hypoxia
• Muscular Dystrophy, Duchenne
• Mitochondrial Damage

• Intestinal Diseases
• Endoplasmic Reticulum Stress
• Ulcer
• Myopathy
• Fibrosis
• Injury To Liver
• Malignant Neoplasms

Interacting Proteins

• ABI2
• BANP
• CMTM5
• TP53

Pathways

• Cell Death
• Necrotic Cell Death
• Pathogenesis
• Methylation
• Programmed Cell Death
• Circadian Rhythm
• Prolactin Secretion
• Mitochondrial Depolarization

• Mating
• Localization
• Secretion

PTMs

• Acetylation

• S-nitrosylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/PPIF

Best Uses Of The PPIF Marker

PPIF is a peptide responsible for catalyzing the cis trans isomerizations of proline imidic peptide peptide chains. PPIase may play an important role in protein folding and regulation and is a useful biomarker in various biological assays. Boster Bio produces antibodies against PPIF in mice and rabbits.

Many biological assays include the use of PPIF-markers

CyP-D is a member of the cyclophilin family and is the equivalent of human CyP-3. It is nuclear-encoded, has a mitochondrial target sequence, and has an unique N terminus. Probes were created to identify murine CyPD cDNAs. The chromosome 14 of mice contains the PPIF genes.

PPIF gene levels are associated to BMP/Smad Signaling in osteogenic Cells. The Ppif gene is regulated by BMP/Smad signaling, which is a direct transcriptional repressor of osteogenic differentiation. Its expression is decreased in osteogenic cells, and increases with BMP2 treatment. However, osteogenic differentiation does NOT necessarily cause pathological calcification. PPIF expression is associated with bone mineralization.

To verify the functionality of Ppif, a negative control was created from the 1.1 kb PPIF construction. Additionally, mitochondrial function assays must be complemented with data on Mt integrity. Smad1 transfection resulted in a 2.5-fold increase of Ppif activity among myogenic cell lines. This suggests that BMP induced hyperactivation in muscle tissue may cause decreased muscle regeneration.

PCR can be used for the isolation of PPIF gene sequences using purified C3H/HeJ mice DNA. Ppif gene fragments are both thicker and thinner PCR fragments. The overlapping region shows homology areas that have been targeted in disruptions of the gene. Different sizes are used to indicate exons and introns. These chimeric male mice then cross with black C57BL/6 mice to test for germ line transmission. Several of these mice passed the Ppif gene to their progeny.

CyP-D also keeps basic regulatory functions in Ppif/ mice. This suggests that mice Ppif/ have a balance between anti- and pro-apoptotic functions. To fully evaluate the phenotype of these mice, further testing will be required in vivo. But the benefits of using Ppif/ mice are many. They are an important tool in biomedical research and clinical diagnosis.

Martin Fussenegger, a professor in Biotechnology and Bioengineering

Dr. Fussenegger is a professor of Biotechnology and Bioengineering at ETH Zurich, Switzerland. He has received numerous awards including the AIChE Bailey Award. Merck Cell Culture Engineering Award. and the Gaden Award. He is a Member of the American Institute of Medical and Biological Engineering and of Swiss Academy of Engineering Sciences.

Professor Fussenegger started his career at ETH Institute of Biotechnology (1996). He was habilitated in 2000 and became the Swiss National Science Foundation Professor in Molecular Biotechnology. He was awarded a chair for Biotechnology and Bioengineering by the ETH Institute for Chemical and Bioengineering. He moved to Basel in 2008 on a presidential mission. His research has lead him to set up a department of engineering and biosystems science at ETH Zurich.

Steven Boster offers high-affinity primary antibodies

Steven Boster Bio is the company you should turn to if your cancer research requires highly specific and sensitive antibody. Boster Bio's antibodies are validated for use in ELISA, WB, immunohistochemistry, and Western Blotting. Over 29,000 publications have used its primary antibodies for PPIF. This antibody collection allows researchers to search for a specific protein using a variety of cancer-related models including tumor cells.