Anti-PPAR alpha/PPARA Antibody Picoband®

PPAR alpha/NR1C1 antibody

Boster Bio Anti-PPAR alpha/PPARA Antibody Picoband® catalog # A00600-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A00600-2
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, WB

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Product Name

Anti-PPAR alpha/PPARA Antibody Picoband®

View all PPAR alpha/NR1C1 Antibodies

SKU/Catalog Number

A00600-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PPAR alpha/PPARA Antibody Picoband® catalog # A00600-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PPAR alpha/PPARA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00600-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PPAR alpha/PPARA recombinant protein (Position: M1-L426).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00600-2 is reactive to PPARA in Human, Mouse, Rat

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Observed Molecular Weight

52 kDa

Calculated molecular weight

52.225kDa

Background of PPAR alpha/NR1C1

Peroxisome proliferator-activated receptor alpha(PPAR-alpha), also known as NR1C1(nuclear receptor subfamily 1, group C, member 1), is a nuclear receptor protein that in humans is encoded by the PPARA gene. PPARA gene spans 83.7 kb and contains 8 exons. And the PPAR gene is mapped to chromosome 22q12-q13.1. Sher et al.(1993) cloned a cDNA for human peroxisome proliferator-activated receptor from a human liver cDNA library. The PPAR cDNA exhibited 85% and 91% DNA and deduced amino acid sequence identity, respectively, with mouse PPAR. PPAR-alpha is a transcription factor and a major regulator of lipid metabolism in the liver.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00600-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -

Positive Control

WB: human HepG2 whole cell, human Hela whole cell, human MCF-7 whole cell, rat liver tissue, mouse liver tissue
FCM: HepG2 cell

Validation Images & Assay Conditions

Gene/Protein Information For PPARA (Source: Uniprot.org, NCBI)

Gene Name

PPARA

Full Name

Peroxisome proliferator-activated receptor alpha

Weight

52.225kDa

Superfamily

nuclear hormone receptor family

Alternative Names

Gap junction alpha-1 protein; Connexin-43; Cx43; Gap junction 43 kDa heart protein; GJA1; GJAL PPARA NR1C1, PPAR, PPARalpha, hPPAR peroxisome proliferator activated receptor alpha peroxisome proliferator-activated receptor alpha|PPAR-alpha|nuclear receptor subfamily 1 group C member 1|peroxisome proliferative activated receptor, alpha|peroxisome proliferator-activated nuclear receptor alpha variant 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PPARA, check out the PPARA Infographic

PPARA infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PPARA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
A00600-2

100μg

$370
A00600-2-10ug

10μg sample (liquid)

$99
A00600-2-Biotin

100 μg Biotin conjugated

$570
A00600-2-Cy3

100 μg Cy3 conjugated

$570
A00600-2-Dylight488

100 μg Dylight488 conjugated

$570
A00600-2-Dylight550

100 μg Dylight550 conjugated

$570
A00600-2-Dylight594

100 μg Dylight594 conjugated

$570
A00600-2-FITC

100 μg FITC conjugated

$570
A00600-2-HRP

100 μg HRP conjugated

$570
A00600-2-APC

100 μg APC conjugated

$670
A00600-2-PE

100 μg PE conjugated

$670
A00600-2-iFluor647

100 μg iFluor647 conjugated

$670
A00600-2-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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A00600-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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