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Product Info Summary
| SKU: | M00600 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC-P, WB |
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Product info
Product Name
Anti-PPARA Antibody
View all PPAR alpha/NR1C1 Antibodies
SKU/Catalog Number
M00600
Size
80 µl
Description
Boster Bio Anti-PPARA Antibody (Catalog # M00600). Tested in IHC-P, IF, Flow Cytometry, WB application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PPARA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00600)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1331CT894.186.143
Isotype
IgG1,κ
Immunogen
This PPARA antibody is generated from a mouse immunized with a recombination protein from the human region of human PPARA.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00600 is reactive to PPARA in Human, Mouse
Reconstitution
Calculated molecular weight
52225 Da
Background of PPAR alpha/NR1C1
Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl- 2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00600 is guaranteed for Flow Cytometry, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:1000
IHC-P: 1:25
FC: 1:25
Validation Images & Assay Conditions
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Fluorescent image of Hela cells stained with PPARA Antibody. M00600 was diluted at 1:25 dilution. An Alexa Fluor® 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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Western blot analysis of lysates from Hela, Jurkat, mouse NIH/3T3 cell line (from left to right), using PPARA Antibody. M00600 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.
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Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using PPARA Antibody. M00600 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Immunohistochemical analysis of paraffin-embedded H. kidney section using PPARA Antibody. M00600 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Flow cytometric analysis of Hela cells using PPARA Antibody(green, Cat#M00600) compared to an isotype control of mouse IgG1(blue). M00600 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For PPARA (Source: Uniprot.org, NCBI)
Gene Name
PPARA
Full Name
Peroxisome proliferator-activated receptor alpha
Weight
52225 Da
Superfamily
nuclear hormone receptor family
Alternative Names
Peroxisome proliferator-activated receptor alpha, PPAR-alpha, Nuclear receptor subfamily 1 group C member 1, PPARA, NR1C1, PPAR PPARA NR1C1, PPAR, PPARalpha, hPPAR peroxisome proliferator activated receptor alpha peroxisome proliferator-activated receptor alpha|PPAR-alpha|nuclear receptor subfamily 1 group C member 1|peroxisome proliferative activated receptor, alpha|peroxisome proliferator-activated nuclear receptor alpha variant 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPARA, check out the PPARA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPARA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PPARA Antibody (M00600)
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