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Key=image_label, value=Western blot analysis of lysates from NCI-H292, A549, HepG2 cell line (from left to right), using ALDH2 Antibody(Cat. #M00546-4). M00546-4 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary anti
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Key=description, value=Boster Bio Anti-PPARA Antibody (Catalog # M00600). Tested in IHC-P, IF, Flow Cytometry, WB application(s). This antibody reacts with Human, Mouse.
Key=short_description, value=Boster Bio Anti-PPARA Antibody (Catalog # M00600). Tested in IHC-P, IF, Flow Cytometry, WB application(s). This antibody reacts with Human, Mouse.
Key=meta_keyword, value=Anti-PPARA Antibody
Key=contents, value=Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Key=background, value= Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl- 2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2.
Key=gene_name, value=PPARA
Key=synonyms, value=Peroxisome proliferator-activated receptor alpha, PPAR-alpha, Nuclear receptor subfamily 1 group C member 1, PPARA, NR1C1, PPAR
Key=application_details, value=IF: 1:25
WB: 1:1000
IHC-P: 1:25
FC: 1:25
Key=subcellular_localization, value=Nucleus.
Key=tissue_specificity, value=Skeletal muscle, liver, heart and kidney. Expressed in monocytes (PubMed:28167758).
Key=protein_function, value=Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn- glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2.
Key=application_notes, value=Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Key=immunogen, value=This PPARA antibody is generated from a mouse immunized with a recombination protein from the human region of human PPARA.
Key=purification, value=This antibody is purified through a protein G column, followed by dialysis against PBS.
Key=storage, value=Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Anti-PPARA Antibody

PPAR alpha/NR1C1 antibody

Boster Bio Anti-PPARA Antibody (Catalog # M00600). Tested in IHC-P, IF, Flow Cytometry, WB application(s). This antibody reacts with Human, Mouse.

Product Info Summary

SKU: M00600
Size: 80 µl
Reactive Species: Human, Mouse
Host: Mouse
Application: Flow Cytometry, IF, IHC-P, WB

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Product Name

Anti-PPARA Antibody

View all PPAR alpha/NR1C1 Antibodies

SKU/Catalog Number

M00600

Size

80 µl

Description

Boster Bio Anti-PPARA Antibody (Catalog # M00600). Tested in IHC-P, IF, Flow Cytometry, WB application(s). This antibody reacts with Human, Mouse.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-PPARA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00600)

Host

Mouse

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Monoclonal

Clone Number

1331CT894.186.143

Isotype

IgG1,κ

Immunogen

This PPARA antibody is generated from a mouse immunized with a recombination protein from the human region of human PPARA.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00600 is reactive to PPARA in Human, Mouse

Reconstitution

Calculated molecular weight

52225 Da

Background of PPAR alpha/NR1C1

Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl- 2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

M00600 is guaranteed for Flow Cytometry, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IF: 1:25
WB: 1:1000
IHC-P: 1:25
FC: 1:25

Validation Images & Assay Conditions

Gene/Protein Information For PPARA (Source: Uniprot.org, NCBI)

Gene Name

PPARA

Full Name

Peroxisome proliferator-activated receptor alpha

Weight

52225 Da

Superfamily

nuclear hormone receptor family

Alternative Names

Peroxisome proliferator-activated receptor alpha, PPAR-alpha, Nuclear receptor subfamily 1 group C member 1, PPARA, NR1C1, PPAR PPARA NR1C1, PPAR, PPARalpha, hPPAR peroxisome proliferator activated receptor alpha peroxisome proliferator-activated receptor alpha|PPAR-alpha|nuclear receptor subfamily 1 group C member 1|peroxisome proliferative activated receptor, alpha|peroxisome proliferator-activated nuclear receptor alpha variant 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PPARA, check out the PPARA Infographic

PPARA infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PPARA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
M00600-80ul

80 μl

$299
M00600-400ul

400 μl

$347

Free Secondary Antibody

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M00600
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

Troubleshooting

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