Anti-Cytochrome P450 Reductase/POR Antibody Picoband®

POR/Cytochrome P450 Reductase antibody

Boster Bio Anti-Cytochrome P450 Reductase/POR Antibody Picoband® catalog # PB9736. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: PB9736
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IHC, ICC, WB

Product Name

Anti-Cytochrome P450 Reductase/POR Antibody Picoband®

View all POR/Cytochrome P450 Reductase Antibodies

SKU/Catalog Number

PB9736

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cytochrome P450 Reductase/POR Antibody Picoband® catalog # PB9736. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cytochrome P450 Reductase/POR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9736)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human POR , different from the related mouse and rat sequences by five amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB9736 is reactive to POR in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

77 kDa

Calculated molecular weight

76690 MW

Background of POR/Cytochrome P450 Reductase

POR is a membrane-boundenzyme required for electron transfer from NADPH to cytochrome P450 in the endoplasmic reticulum of theeukaryotic cell. The gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB9736 is guaranteed for Flow Cytometry, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human

Positive Control

WB: human placenta tissue, human HepG2 whole cell, human CACO-2 whole cell, human K562 whole cell, rat kidney tissue, rat liver tissue, mouse kidney tissue, mouse liver tissue
IHC: Mouse Intestine tissue, Rat Intestine tissue, Human Mammary Cancer tissue
FCM: K562 cell, SiHa cell

Validation Images & Assay Conditions

Gene/Protein Information For POR (Source: Uniprot.org, NCBI)

Gene Name

POR

Full Name

NADPH--cytochrome P450 reductase

Weight

76690 MW

Superfamily

NADPH--cytochrome P450 reductase family

Alternative Names

NADPH--cytochrome P450 reductase;CPR;P450R;1.6.2.4;POR;CYPOR; POR CPR, CYPOR, P450R cytochrome p450 oxidoreductase NADPH--cytochrome P450 reductase|NADPH--hemoprotein reductase|NADPH-dependent cytochrome P450 reductase|P450 (cytochrome) oxidoreductase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on POR, check out the POR Infographic

POR infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for POR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-Cytochrome P450 Reductase/POR Antibody Picoband®

Question

We are currently using anti-Cytochrome P450 Reductase/POR antibody PB9736 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?

A. Jha

Verified customer

Asked: 2013-10-10

Answer

The anti-Cytochrome P450 Reductase/POR antibody (PB9736) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-10-10

Order DetailsPrice
PB9736

100μg

$370
PB9736-10ug

10μg sample (liquid)

$99
PB9736-Biotin

100 μg Biotin conjugated

$570
PB9736-Cy3

100 μg Cy3 conjugated

$570
PB9736-Dylight488

100 μg Dylight488 conjugated

$570
PB9736-Dylight550

100 μg Dylight550 conjugated

$570
PB9736-Dylight594

100 μg Dylight594 conjugated

$570
PB9736-FITC

100 μg FITC conjugated

$570
PB9736-HRP

100 μg HRP conjugated

$570
PB9736-APC

100 μg APC conjugated

$670
PB9736-PE

100 μg PE conjugated

$670
PB9736-iFluor647

100 μg iFluor647 conjugated

$670
PB9736-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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PB9736
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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