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Product Info Summary
| SKU: | A11987-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-POMGNT2 Antibody Picoband®
SKU/Catalog Number
A11987-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-POMGNT2 Antibody Picoband® catalog # A11987-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-POMGNT2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11987-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human POMGNT2 recombinant protein (Position: R40-K378). Human POMGNT2 shares 94.7% and 95% amino acid (aa) sequence identity with mouse and rat POMGNT2, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A11987-1 is reactive to POMGNT2 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
75 kDa
Calculated molecular weight
66.615kDa
Background of POMGNT2
This gene encodes a protein with glycosyltransferase activity although its function is not currently known.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11987-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human 293T whole cell, human PC-3 whole cell, human Jurkat whole cell, human U2OS whole cell, rat brain tissue, mouse brain tissue, mouse pancreas tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of POMGNT2 using anti-POMGNT2 antibody (A11987-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human U2OS whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse pancreas tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POMGNT2 antigen affinity purified polyclonal antibody (Catalog # A11987-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POMGNT2 at approximately 75 kDa. The expected band size for POMGNT2 is at 67 kDa.
Protein Target Info & Infographic
Gene/Protein Information For POMGNT2 (Source: Uniprot.org, NCBI)
Gene Name
POMGNT2
Full Name
Protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2
Weight
66.615kDa
Superfamily
glycosyltransferase 61 family
Alternative Names
Protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2 POMGNT2 AGO61, C3orf39, GTDC2, MDDGA8, MDDGC8 protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-) protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2|glycosyltransferase-like domain containing 2|glycosyltransferase-like domain-containing protein 2|protein O-mannose beta-1,4-N-acetylglucosaminyltransferase 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POMGNT2, check out the POMGNT2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POMGNT2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-POMGNT2 Antibody Picoband® (A11987-1)
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