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Product Info Summary
| SKU: | A30644 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, IF (paraffin) |
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Product info
Product Name
Anti-POLE1 Antibody
View all DNA Polymerase epsilon catalytic subunit A Antibodies
SKU/Catalog Number
A30644
Size
100ul
Form
Liquid
Description
Boster Bio Anti-POLE1 Antibody (Catalog# A30644). Tested in IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-POLE1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30644)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human POLE1. Immunogen sequence location: 1261-1310
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30644 is reactive to POLE in Human, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
261518 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30644 is guaranteed for ELISA, IHC-P, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:40000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using POLE1 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For POLE (Source: Uniprot.org, NCBI)
Gene Name
POLE
Full Name
DNA polymerase epsilon catalytic subunit A
Weight
261518 MW
Superfamily
DNA polymerase type-B family
Alternative Names
POLE;POLE1;DNA polymerase epsilon catalytic subunit A;DNA polymerase II subunit A POLE CRCS12, FILS, IMAGEI1, POLE DNA polymerase epsilon, catalytic subunit DNA polymerase epsilon catalytic subunit A|3-5 exodeoxyribonuclease|DNA polymerase II subunit A|DNA polymerase epsilon catalytic subunit protein|polymerase (DNA directed), epsilon, catalytic subunit|polymerase (DNA) epsilon, catalytic subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POLE, check out the POLE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POLE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-POLE1 Antibody (A30644)
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Customer Q&As
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3 Customer Q&As for Anti-POLE1 Antibody
Question
I see that the anti-POLE1 antibody A30644 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-05-03
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-05-03
Question
We are currently using anti-POLE1 antibody A30644 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-15
Answer
The anti-POLE1 antibody (A30644) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-15
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryonic kidney using anti-POLE1 antibody A30644. Let me know if you need anything else.
S. Roberts
Verified customer
Asked: 2013-11-05
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-11-05
